程计林 程巨才 仝文斌 高巍 马文斌 冯百芳 程计林 仝文斌 高巍 冯百芳 (100044 北京医科大学人民医院肝病研究所)；程巨才 (河南省焦作市化工总厂职工医院) 中华实验和临床病毒学杂志 1999 0 13 3


    关键词：丙型肝炎病毒；单个核细胞 期刊 zhsyhlcbdxzz 0 论著 fur -->


    

【摘要】 目的 复制丙型肝炎病毒(HCV)感染的细胞模型。方法 用EB病毒感染其HCV阳性患者的外周血单个核细胞并使其转化，获得HCV的外周永生化B细胞株。以后，每隔1个月应用套式逆转录聚合酶链反应(RT-PCR)检测1次培养细胞和上清中的HCV RNA。结果 HCV可以在传代细胞中持续存在1年，培养细胞中的HCV负链和培养上清中的HCV则呈间断阳性。结论 HCV可以在该细胞株中较长时间存在、复制和分泌。

Establishment of hepatitis C virus reproductive model of human peripheral blood mononuclear cells transformed by Epstein-Barr virus

CHENG Jilin^* , CHENG Jucai, TONG Wenbin, et al.

    (Institute of Hepatology of People′s Hospital, Beijing 100044)

【Abstract】 Objective For the present, one of the crucial questions to study pathogenic mechanism of hepatitis C and its drug therapy is to seek a suitable cell model which may support the replication of hepatitis C virus(HCV). In this article, we made effort on the study of this aspect.Methods Using Epstein-Barr virus(EBV), we transformed the peripheral blood mononuclear cells(PBMC) isolated from a patient diagnosed as chronic hepatitis C. HCV RNA from the cultured cells and the supernatants were analysed by reverse transcriptase-polymerase chain reaction(RT-PCR) at an intervals of one month.Results HCV positive-strand RNA could be detected continually in the cultured cells for more than one year since the establishment of the transformed PBMC clone whereas the negative-strand RNA were also observed in the cultured cells. Interestingly, the HCV positive-strand RNA could be identified in supernatants intermittently.Conclusion HCV may exist in the cultured cell line for a longer period and reproduce in and secrete out of the cells. This will offer a new cell model for studying the molecular biology and immunology of HCV.

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