关键词：帕金森病；脱噬作用；PC12细胞
摘要 目的 研究帕金森病(PD)黑质多巴胺神经元的死亡机理。 方法 用脱氧核糖核酸(DNA)标记法，借助流式细胞仪和荧光显微镜观察多巴胺(DA)对大鼠嗜铬细胞瘤(PC12)细胞凋亡的影响。 结果 DA可诱发PC12细胞凋亡，适中浓度(0.45mmol/L)时PC12细胞凋亡数最多(凋亡率为48.7%±6.3%)；抗氧化剂二硫苏糖醇(DTT)可部分阻止DA诱发的PC12细胞凋亡(P<0.01)。
结论 细胞凋亡参与了PD的病变过程，采用适当的抗氧化剂可能有益于PD的治疗。
The effect of dopamine on apoptosis in pheochromocytoma cells
Zhou Guoqing , Zhou Xiaoda, Li Ningli, et al. Department of Neurology, Renji Hospital, Shanghai Second Medical University, Shanghai 200001
Abstract Objective To study the mechanism of dopaminergic neuronal death in substantia nigra of Parkinson's disease (PD). Methods Using the DNA end-labeling method the effect of dopamine (DA) on rat apoptotic PC12 cells was examined by flow cytometry and fluorescence microscopy. Results DA induced apoptosis in PC12 cells. Moderate concentration of DA (0.45mmol/L) gave maximal number of apoptotic cells with a rate of apoptosis 48.7%±6.3%; and the antoxidant dithiothreitol (DTT) partially blocked DA-induced apoptosis in PC12 cells (P<0.01). Conclusions Apoptosis may be involved in the pathogenesis of PD, and treatment with proper antioxidants may be useful in PD therapy.
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