【摘要】 目的 研究癌基因的特异性反义RNA对癌细胞生长繁殖和恶性程度的影响。方法 用逆转录病毒载体将人乳头瘤病毒(HPV)-16 E6E7反义RNA导入HPV-16 DNA阳性的宫颈癌细胞株CaSki中，观察该细胞在导入反义RNA后其表型特征和在裸鼠体内致癌能力的变化。结果 HPV-16 E6E7反义RNA能降低宫颈癌细胞CaSki的生长速率，抑制其在软琼脂上的集落形成能力，并能明显地抑制其在裸鼠体内的致癌能力。Western blot分析发现HPV-16 E6E7反义RNA能使宫颈癌细胞中病毒HPV-16 E6基因的表达水平降低。结论 HPV-16 E6E7反义RNA能使宫颈癌细胞CaSki恶性表型逆转；由其引起的癌细胞中HPV-16癌基因表达水平的降低可能是癌细胞表型逆转的原因之所在；HPV-16癌基因的表达水平对维持癌细胞的恶性表型起着重要作用。

Ming Lihua, Zhou Lanping, Shang Ming, et al. Cancer Institute, Chinese Academy Medical Sciences and Peking Union Medical College,Beijing 100021

Abstract In our previous study,the malignant phenotypes of the NIH3T3 cells, transformed with HPV-16 E6E7 genes, could be inhibited by the antisense RNA of HPV-16 E6E7. In this study, the antisense RNA of HPV-16 E6E7 was induced into the cervical carcinoma cells, caski, which contained HPV-16 DNA, The results showed that the cellular growth velocity was down regulated by the antisense RNA of HPV-16 E6E7, the ability of forming cellular colonies in soft agar medium and the tumorigenicity in nude mice of CaSki cells were also inhibited by the antisense cellular colonies in soft agar mediam and the tumorigenicity in nude mice of Caski cells were also inhibited by the antisense RNA. In addition, the decrease of the expression of viral early genes in carcinoma cells was detected with Western blot assay.It denoted that the phenotypes and the carcinogenicity of tumor cells were inhibited by the associated antisense RNA,and this may be one of the pathways inducing tumor cells reversion.

     ......