关键词:肺心病;淋巴细胞;脱噬作用
摘要 目的 研究老年慢性肺心病(CPHD)患者不同病期外周血淋巴细胞(PBL)凋亡的变化,并探讨其临床意义。 方法 用DNA断端标记法(TDT法)测定PBL在体外培养24小时的凋亡发生率,并观察用脂多糖(LPS)和金葡菌肠毒素B(SEB)刺激PBL后24小时凋亡率的变化。结果 患者急性加重期的PBL体外培养24小时后凋亡率(29.94%±11.24%)高于缓解期(11.04%±3.86%)及健康组(9.28%±2.55%),加入LPS及超抗原SEB刺激后,缓解期患者的PBL凋亡率明显增加,与健康组相比差异有显著性。 结论 老年CPHD患者PBL凋亡率与病情有关,应用外源性刺激原可引起PBL的激活诱导凋亡(AICD)。
A study on apoptosis of peripheral blood lymphocytes in aged patients with chronic pulmonary heartdisease Zhu Jian , Tang Jingwei, Li Ningli, et al. * The Ninth People's Hospital, Shanghai Second MedicalUniversity, Shanghai 200011
Abstract Objective To study the apoptosis of peripheral blood lymphocytes(PBL)in aged patients with chronic pulmonary heart disease (CPHD). Methods In situ terminal deoxynuleotidyl transferase-mediated nick-end labeling (TDT) method was used. The PBL were isolated from the venous bloodof the patients during different periods and cocultured with or without LPS or stephylococcal enterotoxin B (SEB)for 24 hours in vitro, then the cultured PBL were labelled by TDT method and identified quantitatively using FACScan. Results Apoptotic
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