【摘要】 目的 检测抗丙型肝炎病毒(HCV)结构区蛋白IgM抗体。方法 采用丙型肝炎病毒C，E1，E2区重组抗原混合包被和分别包被酶标板；用兔抗人γ链血清处理人血清标本，再用固相包被羊抗兔抗体吸附兔抗人γ链-人IgG复合物，建立了抗-HCVIgM检测方法。结果 对76例丙型肝炎病人血清进行抗-HCV IgM检测，同时与逆转录-巢式聚合酶链反应(RT-PCR)检测结果进行比较，两者具有相关性(P<0.005)；IgM抗体组成分析结果表明采用全片段C、C+E2区抗原血清标本中的抗-HCV IgM检出率分别可达96.6%和100%。结论 HCV 的C、E2重组抗原用于抗-HCV IgM检测具有较高的敏感度和特异性。

    Establishment of a method for detecting anti-HCV IgM using recombinated structural region antigens Wang Jian,Min Fuyuan,Zhang Shumin,et al.Beijing Youan Hospital 100054

    Abstract Presence of IgM antibody to viral structurale protein can be taken as the marker of its rdplication,so the detection method for anti-HCV IgM was established using as HCV recombinated structural region antigens,including C,E1,E2,as the coated antigen and rabbit anti-human γ chain sera for neutralization and sheep anti-rabbit IgG coated for the sorbence of the complex of RAH-γ with human IgG.76 serum specimens of the hepatitis C patients were detected by indirect ELISA for anti-HCV IgM and by RT-PCR for RNA. The results suggested that IgM antibodies to C,E1,E2 were closely related with RNA (P<0.005).The components of the IgM antibody,too were identified by indirect ELISA with recombinated antigens coated respectively.When whole-fragment C or C+E2 were used as the coated antigens, anti-HCV IgM in 96.6% or 100% of serum specimens can be respectively detected.Using recombinated structurae region antigens,detection of anti-HCV IgM by ELISA was high sensitivity and specificity.

     ......