刘红兵 张智清 姚立红 侯云德 100052 北京 中国预防医学科学院病毒学研究所病毒基因工程国家重点实验室 中华实验和临床病毒学杂志 1998 0 0 1


    关键词：痘苗病毒；人粒细胞-巨噬细胞集落刺激因子；单纯疱疹病毒；酶联免疫吸附测定 期刊 zhsyhlcbdxzz 0 论著 fur -->


    

【摘要】 目的 观察人GM-CSF(粒细胞-巨噬细胞集落刺激因子)对重组痘苗病毒免疫效果的影响。方法 大鼠体内观察。结果 从核酸和蛋白水平均证实重组痘苗病毒RVJSB1175D/GM-CSF可同时表达GM-CSF及HSV- 1gD(单纯疱疹病毒gD糖蛋白)，但RVJSB1175D/GM-CSF免疫大鼠产生的抗-HSV1gD特异性抗体水平与RVJSB1175D组相比无显著性差异。结论 人GM-CSF在大鼠体内对特异免疫调节作用不明显，可能与GM-CSF主要促使非特异性细胞免疫增强所致有关。

Expression of human granulocyte-macrophage colony stimulating factor(hGM-CSF)by recombinant vaccinia virus and its effect on immunogenicity

Liu Hongbing,Zhang Zhiqing, Yao Lihong, et al. Institute of Virology,Chinese Academy of Preventive Medicine, Beijing 100052

Abstract hGM-CSF was inserted into the down stream of P11K promoter of pJSB1175,which consists of HSV-1gD under the promoter P7.5K.The constructed plasmid pJSB1175D/GM-CSF was cotransfected with vaccinia virus Tiantan Strain into TK-143, After selection and screening, recombinant virus RVJSB1175D/GM-CSF was harvested. It was proved that the recombinant virus could express GM-CSF and HSV-1gD at the same time by MTT assay and IFA in vitro. In vivo study showed that the expression of GM-CSF by RVJSB1175D/GM-CSF did not interfere with the HSV-1gD antibody production.

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