【摘要】 目的 为了研究中国庚型肝炎病毒(HGV)非结构(NS3)区基因结构特征。方法 利用逆转录-半巢式-聚合酶链反应从河南1份HGV RNA阳性血清获得覆盖HGV NS3全长cDNA的4个片段，并克隆到pcDNA Ⅱ载体中，采用Sanger双脱氧末端终止法测定全部cDNA序列。结果 发现克隆到的包括HGV NS3区在内的cDNA序列长度为2137个核苷酸，编码711个氨基酸。与国内外已测定的5侏全序列的相应序列进行同源性比较，其核苷酸同源性在85.7%～93.8%之间，根据核苷酸序列推导的氨基酸序列的同源性为96.1%～97.3%，3株中国HGV NS3区氨基酸存在3个共同变异位点，此外在河南株NS3区还发现了一个与其他5株全序列共同的糖基化位点。结论 河南株HGV NS3区的核苷酸及氨基酸序列相对比较保守，其基因特征的阐明为NS3区基因的生物学功能研究及HGV诊断试剂的研制提供了基础。

    

Abstract To study the genetic characteristics of non-structural region 3 of HGV in China, a HGV-RNA positive sample was collected from Henan. HGV RNA was extracted and amplified by RT-nested-PCR. We got four overlap fragments and they were cloned into plasmid vector pcDNAⅡ. The cDNA was sequenced by the Sanger’s method. Nucleotide homology between Henan strain and other reported HGV isolates (PNF2161, R10291, GBV-C) was 85.7% to 93.8% over the 2137 nucleotides examined. Comparison of amino acid sequence deduced from Henan strain with that of other isolates showed 96.1% to 97.7% homol-ogy. Three amino acid variations were found in NS3 region of all the three Chinese isolates of HGV. In addition, a N-glycosylation site was found in the NS3 region of all the six isolates.

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