刘少山 惠延年 王雨生 王强 710032西安，第四军医大学西京医院眼科(刘少山、惠延年、王雨生)；整形外科(王强) 中华眼底病杂志 1999 0 15 2


    关键词：色素上皮，眼细胞，培养的透明质酸/分析流式细胞术；玻璃体视网膜病，增生性 期刊 zhydbzz 0 增生性玻璃体视网膜病变研究 fur -->


    

【摘要】 目的 观察透明质酸(hyaluronic acid,HA)刺激活性物(HA-stimulating activity,HASA)对培养的人视网膜色素上皮(retinal pigment epithelial，RPE)细胞的作用。 方法 将不同质量浓度的HASA加入RPE细胞培养液，采用活细胞计数、四甲基偶氮唑盐(tetrazolium,MTT)比色法及氚标胸腺嘧啶核苷(³ H-thymidine,³ H-TdR)掺入法检测细胞活力，用流式细胞仪(flow cytometry,FCM)分析RPE细胞周期。 结果 HASA在12.5～200.0 μg/ml范围及 48小时内对人RPE细胞的抑制存在剂量-效应及时间-效应关系。最大细胞抑制率约48.0%。FCM显示HASA作用后G₁ 期细胞数较对照组增加7.2%，S期减少9.7%。 结论 HASA在一定时间和剂量范围内对人RPE细胞有抑制作用。

    中国图书资料分类法分类号 R776 R329.2-332

Inhibition of cultured human retinal pigment epithelial cells by hyaluronic acid-stimulating activity

    LIU Shaoshan,HUI Yannian,WANG Yusheng,et al.Department of Ophthalmology,Xijing Hospital,Fourth Military Medical University,Xian 710032,China

【Abstract】 Purpose To study inhibition effects of retinal pigment epithelial (RPE) cells by hyaluronic acid- stimulating activity(HASA). Methods The cultured human RPE cells added with a series of HASA was measured with cell counting,tetrazolium(MTT)colorimetric assay and tritium-labelled thymidine deoxyribose(³ H-TdR)incorporation assay.Flow cytometry(FCM)analysis was used to examine RPE cells cycles. Results HASA at concentrations of 12.5～200 μg/ml and within 48 hours inhibited RPE cells proliferation with a dose-dependant and time-dependant manners.The maximal inhibition rate of RPE cells by HASF was about 48.0%.FCM revealed that the cells in G₁ phase increased 7.2% and cells in S phase decreased 9.7%,compared to controls. Conclusion HASA at a certain dose range and period can inhibit RPE cells proliferation.

    【Key words】 Pigment epithelium of eye Cell ......