高玲 严密 陈大年 罗成仁 610041 成都，华西医科大学附属第一医院眼科 中华眼底病杂志 1999 0 15 2


    关键词：视网膜变性；基因扩增；序列分析；动物，实验；聚合酶链反应 期刊 zhydbzz 0 论著 fur -->


    

【摘要】 目的 克隆大鼠视网膜缓慢变性/盘膜边缘蛋白(retinaldegeneration slow/peripherin，RDS/peripherin) 基因。 方法 以SD大鼠的视网膜polyA⁺ RNA为模版，采用RT-PCR的方法扩增一段约1 555 bp的cDNA片段，并亚克隆至pBluescriptⅡKS(+)载体中，进行限制性内切酶酶切和序列分析。 结果 证实所克隆的片段是大鼠的RDS/peripherin cDNA，与Begy等报道的序列^[1] 相比，除3′端非翻译区的第1 242位的碱基A→G，第1 409～1 411位碱基CA→CCA外，其它序列基本一致^[1] 。 结论 已获得大鼠RDS/peripherin基因的cDNA，为研究RDS/peripherin基因的功能及在视网膜变性疾病中的作用奠定实验基础。

    中国图书资料分类法分类号 R774.13 R730.43 R392.11

Cloning of rat RDS/peripherin gene

    GAO Ling,YAN Mi,CHEN Danian,et al.Department of Ophthalmology,The First ClinicalMedical College,West China University of Medical Sciences,Chengdu 610041,China

【Abstract】 Objective Molecularcloning of rat retinal degeneration slow(RDS)gene cDNA. Methods UsingPoly A⁺ RNA from retina of SD rat as template,a 1 555bp positive cDNAband was obtained by RT-PCR and subcloned into pBluescriptⅡKS(+) vector.The clonedfragment was analyzed with restriction endonucleases and sequencing. Results Ithad been proved that the cloned fragment was rat RDS/peripherin cDNA.Except for thesubstitute of A1 242G and CA 1409-1 411CCA,the other sequences corresponded to that reportedby Begy. Conclusion Rat RDS/peripherin cDNA was obtained.Researcheson function of rat RDS/peripherin gene and its role in retinal degeneration are underway.

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