关键词：眼损伤，穿透性/药物疗法；玻璃体视网膜病，增生性/药物疗法；维拉帕米/治疗应用；疾病模型，动物
【摘要】 目的 观察维拉帕米(verapamil,Ver)对眼后节穿通伤增生性玻璃体视网膜病变(proliferative Vitreoretinopathy,PVR)的影响。方法64只青紫蓝兔，每只兔任选其中一只眼制备外伤性PVR模型后随机分为两组，治疗组在模型制备后立即每日结膜下注射2.5 mg/ml的Ver0.5ml，对照组则每日结膜下注射生理盐水0.5ml，共21天。模型制备后7天内每天用裂隙灯显微镜及间接立体检眼镜观察伤口、角膜、前房及眼底情况，此后每周第3，7天各检查1次。伤后第35天时常规摘除模型制备眼作光镜观察。结果 在模型制备后第35天，治疗组18只眼发生牵引性视网膜脱离，占56.3%，对照组26只眼发生，占81.3%(χ2 =4.655，P=0.031)；玻璃体视网膜增生程度治疗组较对照组明显减轻(秩和检验，u=3.5419，P<0.001)；未发现Ver对角膜上皮有明显毒性反应。 结论 结膜下注射Ver能有效抑制外伤性PVR的发展，减少牵引性视网膜脱离的发生率；Ver在实验所用剂量对角膜上皮无明显毒性反应。
中国图书资料分类法分类号 R774.1 R770.5 R965.1
The therapeutic effects of subconjunctival verapamil on experimental traumatic proliferative vitreoretinopathy
JIANG Caihui,ZHANG Maonian,ZHANG Kun,Department of Ophthalmology,the General Hospital of PLA,Beijing 100853,China.
【Abstract 】 Objective To investigate the therapeutic effects of subconjunctival verapamil on outcome in an experimental model of traumatic proliferative vitreoretinopathy. Methods An experimental model of traumatic proliferative vitreoretinopathy was induced in pigment rabbits,which then were selected randomly to receive either subconjunctival verapamil injection treatment or a placebo injection(control)daily for 3 weeks.Animals were examined by indirect ophthalmoscopy at weekly intervals for 5 weeks.Eyes were enucleated for light microscopy 5 weeks later. Results Fifty-six percent(18 of 32)of the rabbits receiving subconjunctival verapamil injection had developed tractional retinal detachment,whereas eighty-one percent(26 of 32)of control animals had developed tractional retinal detachment(χ2 =4.655,P=0.031).The results of clinical examination and light microscopy didnt show evidence of toxicity between the verapamil treated animals and control animals. Conclusion Subconjunctival verapamil decreased the incidence of tractional retinal detachment due to traumatic proliferative vetreoretinopathy in this rabbit model.Verapamil at the dose used in this model has no evident toxicity on rabbit eyes.Further studies are needed to determine the doseresponse and efficacy of the drug.
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