【摘要】 目的 试图用肝癌细胞体外培养试验,推测肝细胞表达CD95配体(CD95L)是否能自身通过凋亡来清除乙型肝炎病毒(HBV)。方法 诱 导HepG2细胞表达CD95L,与转染HBV、表达CD95的HepG2.2.15细胞共培养,以荧光显微镜和流式细胞仪检测后者的凋亡。结果 表达CD95L的HepG2细胞杀伤表达CD95的HepG2.2.15细胞, 24小时凋亡16.5%, 48小时凋亡43.0%, 这一作用可用抗-CD95L阻断。结论 乙型肝炎时，可能肝细胞自身经非免疫机制清除病毒。

Clearance ofintrahepatic hepatitis B virus by a nonimmune mechanism

ZHU Youfu, LUO Kangxian, WANG Jinyu. Department of Infectious Diseases, Nanfang Hospital,Guangzhou 510515

    【Abstract】 Objective The clearance of intrahepatic hepatitis B virus(HBV) is mainly by the cytotoxicity of immune cells with ways includingCD95ligand/CD95 induction. We found that the liver cells do express CD95 ligand(CD95L), acytotoxic cellsurface molecule. This paper studied the mechanism and implications of thehepatocytes themselves to clear the virus. Methods The HepG2 cells withinduced CD95L and the HepG2.2.15 cells with constitutive CD95 expression were coincubated.The apoptotic cells were examined under fluorescene microscope and with flow cytometry. Results In chronic hepatitis B intrahepatic expressions of CD95L and/or CD95were detected at both mRNA and protein level. Methotrexate induced CD95L expression ofHepG2 cell. The HepG2 cells with CD95L could kill the HepG2.2.15 cells with CD95 moleculewith apoptotic rates of 16.5% at 24h and 43.0% at 48h. This apoptosis effect could beblocked by using anti-CD95L antibody. Conclusion T he hepatocyte mightinduce apoptosis mutually and clear the virus themselves with a nonimmune mechanism.

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