【摘 要】 目的 探索格林-巴利综合征(GBS)的发病机制与空肠弯曲菌(CJ)的关系以及我国GBS病前CJ感染率比较高的原因。方法 用改良的Skirrow方法在1995～1997年收集的47例GBS患者的粪便标本中分离CJ，以Penner方法进行血清学分型，对不能分离出CJ的患者粪便标本进行聚合酶链反应(PCR)-RFLP分型。对同期171例CJ肠炎患者的CJ菌株用Penner方法进行血清学分型。结果 共分离出6株CJ菌株，其中Penner2型3例、19型1例、4型1例、26型1例。同期分离的171例CJ肠炎患者的CJ菌株常见的血清型为Penner2、19和8型。PCR-RFLP分型发现3株DNA-Ⅰ型，相当于Penner 19型。结论 我国引起GBS的CJ血清型和国外报告的相似，并且诱发GBS的血清型与CJ肠炎患者的血清型有极大相似性，这可能是GBS发病与CJ肠炎关系密切的原因。而Penner26型也是引起GBS的血清型之一。

The relationship betweenGuillain-Barre syndrome and serotypes

    of Campylobacter jejuni isolated from the patients

    LI Haifeng, YUAN Jinmei, SHEN Baoquan, et al.

    The First Hospital, Beijing Medical University, Beijing 100034

【Abstract 】 Objective Toinvestigate the relationship between Campylobacter jejuni (CJ) serotype and the highincidence of Guillain-Barre syndrome (GBS) following CJ enteritis in China. Methods CJwas isolated from fresh stool of 47 GBS patients in Beijing area from 1995-1997 with acombination of Skirrow method and micro-filtration method. Serotpying of the CJ strainswas performed with passive hemagglutination method with standard serum from CDC. 171 CJstrains from CJ enteritis in &127;our hospital in the same period were serotyped with thesame method. The stool from which no CJ could be isolated was typed with PCR-RFLP method. Results 3Penner 2, 1 Penner 4, 1 Penner 19 and 1 Penner 26 strains were isolated and serotyped.Three strains which could not be isolated were found by PCR-RFLP method to be DNA-I, whichis equivalent to the type of Penner 19. There were some CJ strains which could be typedwith serum from CDC. Conclusion CJ from GBS patients in Beijing areaare similar to those reported in the world; it is suggested that CJ may cause GBS bymolecular mimicry mechanism in China. The most common serotype of CJ from GBS was reportedto be Penner 19 in Japan, while it was rare in CJ enteritis. However, the similarity ofthe serotypes between CJ from GBS patients and CJ from enteritis patients may explain thehigh incidence of GBS following CJ infection in China. Penner 26 is reported to beisolated from GBS patients for the first time.

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