曹彬 郭子健 许文兵 朱元珏 100730 中国医学科学院、中国协和医科大学 北京协和医院呼吸科 中华内科杂志 1999 0 38 12


    关键词：肺纤维化；胰岛素样生长因子Ⅰ；血小板衍生生长因子；血管发生 期刊 zhnkzz 0 论 著 fur -->


    

【摘要 】 目的 通过研究特发性肺间质纤维化(IPF)患者开胸肺活检标本中胰岛素样生长因子(IGF)-Ⅰ和血小板衍生生长因子(PDGF)的表达，进一步阐明它们在IPF过程中的作用。方法 采用免疫组化和原位杂交方法，分别利用IGF-Ⅰ和PDGF的特异抗体和特异引物，检测其在IPF患者开胸肺活检标本中的分布和表达。结果 在IPF患者中，IGF-Ⅰ主要分布在肺动脉血管、新生血管的平滑肌细胞和内皮细胞。肺泡巨噬细胞、Ⅱ型上皮细胞和间质细胞染色阳性。PDGF定位于增生的细支气管、Ⅱ型肺泡上皮细胞和间质成纤维细胞，肺泡巨噬细胞、血管内皮和平滑肌细胞也有较强着色。结论 IGF-Ⅰ和PDGF可促进新生血管的产生及肺血管平滑肌细胞肥大、增生，在IPF进程中起重要作用；同时，通过与肺实质细胞的相互作用，参与上皮细胞增生和修复、间质细胞增生和胶原沉积。

The potential role of cytokine expressionand its relation to angiogenesis in idiopathic pulmonary fibrosis

    CAO Bin, GUO Zijian, XU Wenbing, et al.Department of Respiratory Diseases, Peking UnionMedical College Hospital, Peking Union Medical College ,Chinese Academy of MedicalScience, Beijing 100730

【Abstract 】 Objective Theexpression of insulin-like growth factor(IGF)-Ⅰ and platelet-derived growth factor (PDGF) in open lung biopsies from patients with idiopathic pulmonary fibrosis (IPF) wasstudied to investigate their precise role in the development of fibrosis in IPF. Methods Thedistribution and expression of IGF-Ⅰ and PDGF were examined in lung sections of IPFpatients with antibodies and cDNA probes of IGF-Ⅰ and PDGF by immunohistochemistry andin situ hybridization. Results IGF-Ⅰ was predominantly localized invascular smooth muscle cells and vascular endothelial cells of newly formed vessels in IPF. Smooth muscle cells of pulmonary arteries which underwent hypertrophy and hyperplasiastrongly expressed IGF-Ⅰ(+++). Type Ⅱ cells, alveolar macrophages, and interstitialcells were also stained with anti-IGF-Ⅰ, but with lesser intensity (+). PDGF-AA and BBwere localized in hyperplastic bronchio-alveolar epithelial cells and interstitialfibroblasts (+++). Alveolar macrophages, vascular endothelial cells and smooth musclecells were also stained positive (++). Conclusion IGF-Ⅰ and PDGFpromote angiogenesis and vascular smooth muscle cell hypertrophy and hyperplasia, whichcontribute to the progression of fibrosis in IPF. IGF-Ⅰand PDGF are also involved inreepithelization and fibroproliferation in IPF through interaction with mesenchymal cells.

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