房静远 萧树东 陈向荣 童菊芳 200001 上海第二医科大学附属仁济医院、上海市消化疾病研究所 中华内科杂志 1998 10 37 10


    关键词：胃肠肿瘤；脱噬作用；叶酸 期刊 zhnkzz 0 论 著 fur -->


    

【摘要】 目的 探讨不同分化程度的胃癌细胞凋亡情况和全反式维甲酸对其影响。方法 以人胃癌细胞系低分化的MKN-45和高分化的MKN-28细胞作为研究对象，以不同浓度的全反式维甲酸干预其72小时后，以原位DNA断段切口标记法和DNA琼脂糖电泳检测细胞凋亡情况。结果 两种细胞系的凋亡率均低于5%，全反式维甲酸干预后凋亡指数上升，且可能与药物浓度有关。结论 全反式维甲酸可诱导胃癌细胞凋亡。

    Induction of apoptosis with folic acid in human gastric cancer cell lines MKN-45and MKN-28 Fang Jingyuan, Xiao Shudong, Chen Xiangrong, et al. ShanghaiInstitute of Digestive Diseases, Shanghai Second Medical University, Shanghai 200001

    【Abstract】 Objective To induce gastric cancer cellapoptosis with folic acid and to evaluate its anticarcinogentic mechanism. Methods Theability of folic acid to induce the onset of gastric cancer cell apoptosis wasinvestigated by using human gastric cancer cell lines MKN-45 and MKN-28 and the DNAfragmentation was studied in situ by TdT-mediated dUTP-biotin nick end labeling and DNAagarose gel electrophoresis. Results The rates of apoptosis in bothpoorly differentiated MKN-45 and well differentiated MKN-28 cell lines were less than 5%.Apoptosis could be induced by administration of all trans-retinoic acid and apoptosisindices of MKN-45 and MKN-28 cells were related to dosage of the drugs. Conclusion Itis concluded that induction of gastric cancer cell apoptosis by folic acid may play a rolein the anticarcinogenesis, so it may possibly be used as a therapeutic agent in thetreatment of human gastric cancer.

     ......