徐卫东 吴岳嵩 张春才 侯铁胜 胡惠民 毛积芳 颜永碧 200433上海，第二军医大学长海医院骨科(徐卫东、吴岳嵩、张春才、侯铁胜)；第二军医大学生物化学教研室(胡惠民、毛积芳),电镜中心实验室(颜永碧) 中华骨科杂志 1999 0 19 4


    关键词：转化生长因子β成纤维细胞生长因子免疫组织化学 期刊 zhgkzz 0 基础研究 fur -->


    

【摘要 】目的观察成纤维细胞生长因子对培养兔关节软骨细胞转化生长因子-β1的表达。方法培养1月龄新西兰兔的关节软骨细胞，在每次换液时加入成纤维细胞生长因子100ng/ml，铺满后收集细胞，作关节软骨细胞转化生长因子-β1常规及电镜免疫组织化学研究。结果光镜下实验组细胞明显呈棕色，电镜下细胞膜表面有明显的胶金颗粒粘附。结论成纤维细胞生长因子能促进关节软骨细胞转化生长因子-β1的表达。

    The Study of theImmunohistology of Rabbit Articular Chondrocytes

    Transforming Growth Factor β1

XU Weidong,WU Yuesong,ZHANG Chuncai，et al.

    Department of Orthopaedics,Changhai Hospital,The Second Military Medical University，Shanghai200433

【Abstract 】Objective To observe theexpression of transforming growth factor β1(TGF-β1)of the cartilage cells with thefibroblast growth factor(FGF).Methods The rabbit articular chondrocytes werecul-tured,while the FGF(100 ng/ml)were put into the cell culture medium.After the growthwas consistent,the chondrocytes were harvested.Then the immunohistology of TGF-β1 onthe cartilage cells were performed to understand the interrelation and interaction of thegrowth factors.Results The study showed that the chondrocytes treated with the FGF(100 ng/ml)had an obvious brown color,with the membranes adhered with a large number of grainsof colloid-gold under electron microscope.Conclusion This study indicated that the FGFcould prompt the expression of TGF-β1 of the cartilage cells.

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