田健 汤钊猷 叶胜龙 刘银坤 林芷英 200032 上海医科大学中山医院肝癌研究所 中华肿瘤杂志 1998 11 0 6


    关键词：肝肿瘤；癌，肝细胞；肿瘤细胞，培养的；肿瘤转移；甲胎蛋白；染色体 期刊 zhzlzz 0 基础研究 fur -->


    

【摘要】 目的 利用裸鼠人肝癌高转移模型(LCI-D20)的皮下移植瘤组织在体外建立一株具有高转移潜能的人肝癌细胞系(MHCC97)，并对其一般生物学特性进行观察。方法 将分离的瘤细胞制成细胞悬液，用10%人AB型血清的高糖DMEM培养液建成该细胞系，采用流式细胞术和染色体G-显带方法，进行细胞遗传学分析；用ABC免疫组化法，观察其肺转移灶中癌细胞甲胎蛋白(AFP)表达情况。结果 MHCC97细胞为典型的上皮样细胞，符合一般上皮性恶性肿瘤细胞的病理学特征。该细胞经皮下和肝内接种均可使裸鼠致瘤，并发生肺部转移。肝内接种者，肺转移达100%(12/12)。MHCC97细胞为异倍体细胞，染色体均为超二倍体，i(1)(q)和der(4)(pter→q35::?)等为其标志染色体，未显示有完整Y染色体存在。肺转移灶的癌细胞AFP阳性。结论 MHCC97细胞具有与原移植瘤相似的生物学特性。染色体的畸变可能与其发生发展有关。

Establishment of a humanhepatocellular carcinoma(HCC) cell line with high metastatic potential (MHCC97) and itsbiological characteristics Tian Jian, Tang Zhaoyou, Ye Shenglong, et al. Liver CancerInstitute, Zhongshan Hospital, Shanghai Medical University, Shanghai 200032

     【Abstract】 Objective To establish and characterize a human hepatocellular carcinoma cell line withhigh metastatic potential derived from a subcutaneous xenograft of metastatic human HCC innude mice (LCI-D20). Methods Single-cell suspension collected by tearing tumor tissueswith forceps was transferred to the tissue culture flasks for culture in vitro in mediumDMEM supplemented with 10% human group AB serum. Cytogenetic studies were performed onthis cell line using flow cytometry and chromosome G-banding. AFP of theprimary xenograftsand lung metastatic lesions was detected by using ABC immunohistochemistry. The rates ofits tumorigenicity and metastasis in nude mice were evaluated. Results T he MHCC97 cells showedtypical epithelial appearance. Upon subcutaneous or intrahepatic inoculation in nude mice,the xenograft grew and metastasized to the lungs. The metastatic rate was 100%. The cancercells of lung metastatic foci were AFP positive. Aberrant chromosomes i(1)(q) and der(4) (pter→q35::?) wereits chromosome markers. Conclusion The MHCC97 cell line maintained the biologiccharacteristics as its original xenografts. The presence of the reported chromosomalaberrations may be related to carcinogenesis and progression of HCC.

     【 Subject words】 Liver neoplasms Carcinoma,hepotocellular Tumor cells, cultured Neoplasm metastasis Alpha-fetoproteins Chromosome

原发性肝癌已成为我国最常见的恶性肿瘤之一 ......