武利存 张维东 刘惠萍 赵斌 孙公甲 武利存 张维东 孙公甲 250062 济南市，山东省医学科学院基础医学研究所；刘惠萍 济南市中心医院病理科；赵斌 山东医科大学校医院 中华肿瘤杂志 1999 0 21 6


    关键词：乳腺肿瘤；淋巴结转移；血管生成；免疫组织化学 期刊 zhzlzz 0 临床研究 fur -->


    

【摘要】 目的 研究乳腺癌腋窝淋巴结转移的血管生成情况。方法 采用内皮细胞ⅧF-RAg免疫组化染色技术，对37例乳腺癌根治术或改良根治术切除的乳腺癌组织和121枚腋窝转移淋巴结进行免疫组化染色。在100倍视野下通过显微电视系统计数微血管密度(MVD)，并用显微测量器测量转移灶的直径。结果 在121个淋巴结中找到13处微转移灶，其平均直径为(210±37)μm，无血管生成。腋窝淋巴结转移瘤的MVD为89.3±18.4，与乳腺癌组织MVD(93.8±21.8)差异无显著性，且微血管分布不均，周围高于中央。结论 淋巴结微转移灶无血管生成，转移瘤有血管生成。为抑制微转移灶发展成转移瘤，以及抑制转移瘤的生长，抑制血管生成可能是控制淋巴结转移的有效措施。

Immunohistochemicalstudy of angiogenesis of axillary lymph node metastasis in human breast carcinoma

WU Licun, ZHANG Weidong, LIU Huiping, et al. Institute of Basic MedicalSciences, Shandong Academy of Medical Sciences, Jinan 250062

【Abstract】 Objective To study angiogenesisof axillary lymph node metastases (including micrometastatic foci and metastatic tumors)in breast carcinoma and the relationship between microvessel density (MVD) and metastasis.Methods Thirty-seven breast cancer tissues and 121 metastatic axillarylymph nodes were collected from breast cancer patients and studied immunohistochemically.MVD was counted by means of microvideo system under 100×magnification. The diameter ofeach micrometastasis was measured with a micrometer.Results The meandiameter of 13 micrometastatic foci was 210±37 μm. No blood vessel formation was found.MVD of the primary tumor and that of metastatic tumor in the axillary lymph node was 93.8±21.8and 89.3±18.4, respectively (P>0.05). The disribution of microvessels of the metastatictumor in the lymph node and that of the primary tumor was similar, being higher at theperiphery than at the center.Conclusion Micrometastatic foci of breastcarcinoma in the axillary lymph node do not have new blood vessel formation. Their furthergrowth, however, depends on neo-angiogenesis. Treatment based on inhibition ofangiogenesis may be efficacious in the prevention of micrometastatic foci from developinginto metastatic tumor in lymph node.

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