阳剑波 唐湘娜 邓龙文 谭国林 周鸣 曾朝阳 曹莉 李桂源 410078 长沙，湖南医科大学肿瘤研究所 中华肿瘤杂志 1999 0 21 6


    关键词：鼻咽肿瘤；染色体9p21～22；杂合性丢失；肿瘤抑制基因 期刊 zhzlzz 0 基础研究 fur -->


    

【摘要】 目的 进一步精细限定鼻咽癌9p21～22区域等位基因杂合性丢失的频率和范围，为发现和分离克隆该区域内的鼻咽癌抑瘤基因提供新线索和依据。方法 应用11个定位于9p21～22区域的高密度微卫星位点，检测25例低分化鼻咽癌患者的杂合性丢失。结果 25例患者中，有17例存在一个或多个位点的杂合性丢失，占68.0%。其中D9S161(35.0%)、D9S1678(31.6%)、D9S263(33.3%)和D9S1853(33.3%)4个紧邻位点的丢失频率相对较高，并发现有6例患者在这些位点表现为连续性缺失。结论 鼻咽癌染色体9p21～22区域D9S161～D9S1853位点之间(2.7 cM)是鼻咽癌患者的一个较小共同缺失区，该区域内的抑瘤基因失活可能是鼻咽癌发生发展过程中的重要事件。

Detailed deletionmapping of chromosome 9p21～ 22 in nasopharyngeal carcinoma

YANG Jianbo, TANG Xiangna, DENG Longwen, et al.Cancer Research Institute, Hunan Medical University, Changsha 410078

【Abstract】 Objective To further refine theextent of deletion on chromosome 9p21～22 in nasopharyneal carcinoma(NPC) and provideevidence for discovering new tumor suppressor genes.Methods Loss ofheterozygosity (LOH) on chromosome 9p21～22 was analyzed in 25 paired blood and tumorsamples by using 11 high-density microsatellite polymorphic markers. Results Ofthe 25 cases, 17(68.0%) showed LOH at one or more loci. Higher frequencies of LOH werefound at four loci: D9S161(35.0%), D9S1678(31.6%), D9S263(33.3%) and D9S1853(33.3%). In 6cases, contiguous stretch of allelic loss was found.Conclusion Theminimal common region of deletion might be defined between D9S161 and D9S1853(approximately 2.7 cM in length) at 9p21.1, suggesting that inactivation of one or moretumor suppressor genes located in this region may be an important step in NPC.

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