古柏燕 任红 古柏燕 任红(400010 重庆医科大学病毒性肝炎研究所) 中华肝脏病杂志 1999 0 7 2


    关键词：乙肝表面抗原 载体 真核表达 期刊 zhgzbzz 0 基础研究 fur -->


    

【摘要 】目的 建立HBsAg及其变异体的高效真核表达系统。方法 从已构建的pBluescriptKS⁺ -HBs系列突变重组体获取HBV-S基因片段，将其亚克隆到真核表达载体pMEP₄ ，pCEP₄ ，pLXSN，PXT₁ 及pcDNA₃ ，从而构建了含HBV-S基因及其突变型的一系列表达载体，并将其转染(或感染)人肝癌细胞系HepG₂ 。结果 获得稳定分泌HBsAg及其突变体的抗性细胞系。结论 各组真核表达载体皆能表达HBsAg及其突变体，但在转染率、表达量及稳定性上存在差异。选择其中的高效、准确表达系统，将为HBsAg变异株生物学性状的深入研究及进一步基因治疗、基因免疫的研究提供有用的工具。

    

Expression of HBsAg by usingvarious eukaryotic expression vectors

GU Baiyan, REN Hong.

    Chongqing Medical University, Chongqing 400010

【Abstract 】 Objective To establish efficient eukaryotic expression sysytem of HBsAg and its variants. Methods A series of expression vectors were constructed by cloning HBV S gene and its mutant genesfrom recombinant vector pBluescript pBKS⁺ -HBs containing S gene and its mutantgenes into eukaryotic expression vectors: pMEP4, pCEP₄ , pLXSN, PXT₁ ,pcDNA₃ . These recombinant eukaryotic expression vectors were transfected intohuman hepatocellular carcinoma cells (HepG₂ ). Results The celllines expressing recombinant proteins were stably established. Conclusion All expression vectors could express HBsAg and its variants, but their transfectingefficiency, expressing efficiency and stability of expression were different. Theestablishment of highly efficient expression system in this study paves the way forfurther study on biologic characters of HBsAg variants, genetic immunization and genetictherapy.

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