张慧 冯作化 张桂梅 李东 430030 武汉，同济医科大学实验医学研究中心分子生物学研究室 中华肿瘤杂志 1999 0 21 6


    关键词：纤维连接蛋白；重组多肽 期刊 zhzlzz 0 基础研究 fur -->


    

【摘要】 目的 研究纤维连接蛋白双结构域重组多肽(CH50)抑制体内肿瘤细胞形成转移灶的作用。方法 采用皮下和腹腔接种黑色素瘤B16/F1细胞，在治疗后测定肿瘤大小及转移灶数量。结果 对于皮下接种肿瘤，局部注射较远距离注射更为有效，对肿瘤生长抑制可达50%；对腹腔内形成的微小肿瘤转移灶的抑制达 80%；对>1 mm的转移结节的抑制率也可达到50%以上；与化疗药物羟基喜树碱合用则具有更强的疗效。结论 CH50对微小肿瘤灶的形成具有很强的抑制作用，同时也可以有效抑制较大肿瘤结节的增长，有望成为肿瘤综合治疗中的一种重要成分。

The inhibitoryeffect of recombinant fibronectin polypeptide CH50 on the growth of B16 melanoma

ZHANG Hui, FENG Zuohua, ZHANG Guimei, et al. Department of MedicalMolecular Biology, Tongji Medical University, Wuhan 430030

【Abstract】 Objective To study theinhibitory effect of CH50, a recombinant polypeptide consisting of 2 binding domains ofhuman fibronectin, on the growth of B16/F1 melanoma in mice.Methods Miceinoculated with B16/F1 cells were treated with CH50. The growth inhibitory effect on ipinoculated tumor was assessed by the number and size of tumor nodules in the peritonealcavity, and on sc inoculated tumor by tumor size.Results For scinoculated B16/F1, CH50 injected at the tumor site led to 50% inhibition of tumor growthwhile injection at a distant site was much less effective. In mice bearing ip inoculatedtumor, the growth inhibition by CH50 on tumor nodules <1 mm in diameter was 80% and 50⁺ %on those >1 mm. Synergistic therapeutic effect was obtained when CH50 used incombination with hydroxycamptothecine (HCPT).Conclusion CH50 iseffective in the treatment of B16/F1 melanoma. The effect can be enhanced when used incombination with HCPT.

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