付晓颖 张颂文 冉瑞琼 申宗候 顾建新 曹世龙 200032 上海医科大学肿瘤医院放疗科(付晓颖、冉瑞琼、曹世龙)；上海医科大学基因中心(张颂文、申宗侯、顾建新) 中华肿瘤杂志 1999 0 0 2


    关键词：p16基因；基因治疗；细胞周期；肺肿瘤；腺癌 期刊 zhzlzz 0 基础研究 fur -->


    

【摘要】 目的 探讨p16基因对人肺腺癌的作用及p16基因治疗肺腺癌的可能性。方法 运用分子克隆技术构建重组人p16基因表达载体，以电穿孔法将p16基因导入到该基因缺失的wtp53型的人肺腺癌A549和H460细胞中，得到稳定表达p16的人肺腺癌细胞。详细研究p16基因对wtp53型人肺腺癌细胞周期的影响和细胞增殖的作用。结果 导入p16基因能使人肺腺癌A549和H460细胞生长速度较对照细胞明显减慢，细胞周期阻滞在G₁ 期，克隆形成率下降，致瘤性减少。结论 导入外源性p16基因能抑制人肺腺癌细胞的增殖。

Effect of exogenous p16 gene on the growth of wild-type p53 humanlung adenocarcinoma cells FU Xiaoying, ZHANG Songwen, RAN Ruiqiong, et al. CancerHospital, Shanghai Medical University, Shanghai 200032

    【Abstract】 Objective To study the effect of p16 gene onhuman adenocarcinoma cell lines and the posibility of exogenous p16 gene for gene therapyof lung cancer. Methods A p16 gene recombinant expression vector wasconstructed by cloning p16 cDNA into the pcDNA3 vector at the sites of BamH1 and Xho I,and was transferred by electroporation into p16 gene deficient human lung adenocarcinomacell lines (A549 and H460)which expressed wild-tyre p53 gene.The p16 mRNA and proteinexpression of A549-p16 and H460-p16 cell was detected by Northern blots and immunocytochemistry, respectively.The cell growth properties and cell cycle pattern wereaassessed by flow cytometry. Results The growth and colony formingefficiency of A549-p16 and H460-p16 cells were significantly inhibited compared with thecontrols. Flow cytometry showed A549-p16 and H460-p16 cells were arrested in G1 phase ofcell cycle, and the percentage of cells in S phase and G2/M phase was decreasedsignificantly. Tumorigenicity of the A549-p16 and H460-p16 cells in nude mice was greatlyinhibited. Conclusion The results show that human p16 gene can suppressthe malignant phenotype of wild-type p53 human lung adenocarcinoma cell lines (A549 andH460). p16 gene can play a role in the gene therapy of human lung adenocarcinoma.

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