陈红松 丛旭 王宇 100044 北京医科大学人民医院肝病研究所 中华肝脏病杂志 1998 0 0 3


    关键词：TT病毒 血液透析 基因分析 期刊 zhgzbzz 0 临床研究 fur -->


    

【摘要】 目的 研究我国血液透析患者人群中经输血传播病毒(TTV)的感染及基因变异状况。方法 设计特异性引物采用半巢式PCR方法对北京市2所综合性大医院50例血液透析患者血清标本进行TTV DNA的检测并将产物克隆及序列分析。结果 经过半巢式PCR检测，血透患者TTV DNA阳性率为14%。对其中4例标本中分离出的TTV270个碱基核酸片段序列分析表明：基因同源性为99.1%～100%，推测的编码氨基酸序列同源性为100%。与国外报道的日本株比较，核苷酸同源性为88.3%～97.8%，推测的编码氨基酸同源性为96.1%～98.7%。TTV阳性与阴性组转氨酶升高的患者比例差异无显著性。结论 我国血透患者中存在较严重的TTV感染，其病毒部分基因及推测编码蛋白十分保守，但与日本所报道的TTV毒株有明显差别。未见TTV感染与转氨酶升高密切相关的证据。

    

INFECTION AND GENETICANALYSIS OF TRANSFUSION TRANSMITTED VIRUS (TTV) IN HEMODIALYSIS PATIENTS IN BEIJING

Chen Hongsong, Cong Xu, WangYu. Institute of Hepatology, People's Hospital, Beijing Medical University, Beijing 100044

【Abstract】 Objective To study the infection and genetic variation oftransfusion transmitted virus(TTV) in hemodialysis patients. Methods 50hemodialysis patients from Beijing were studied. DNAs of TTV were amplified by semi-nestedpolymerase chain reaction (PCR) with specific primers. The PCR products were cloned andsequenced. Results 14% of hemodialysis patients were positive for TTVusing semi-nested PCR. 270bp fragment of TTV DNA were isolated from 4 patients. Among 4isolates of TTV (T001～T004) the gene sequencehomologies were above 99.1% and the deduced amino acid sequence homologies were 100%. Thecomparison of these 4 isolates and 4 Japanese isolates reported previously showed that thegene sequence homologies were in the range from 88.3% to 97.8% and deduced amino acidsequence homologies were from 96.1% to 98.7%. There were not significant differences inthe prevalence of transaminase (ALT) elevated between hemodialysis patients with positiveand negative of TTV marker. Conclusion TTV infection is found in China.The gene and deduced amino acid sequences of TTV are highly conserved among isolates inthis study.

     ......