荆绪斌 李定国 陆汉明 515031 汕头大学医学院附属第一医院(荆绪斌)；上海第二医科大学附属新华医院(李定国、陆汉明) 中华肝脏病杂志 1998 0 0 3


    关键词：贮脂细胞 肝纤维化 钙调蛋白拮抗剂 期刊 zhgzbzz 0 基础研究 fur -->


    

【摘要】 目的 探讨钙调蛋白拮抗剂抗肝纤维化作用的机制。方法 采用链酶蛋白酶、胶原酶及密度梯度离心的方法，分离、培养了大鼠肝脏贮脂细胞，用四甲基偶氮唑蓝(MTT)比色法及3H-脯氨酸掺入的方法，观察了钙调蛋白拮抗剂对贮脂细胞增殖及胶原合成的影响。结果 低浓度钙调蛋白拮抗剂对贮脂细胞增殖及胶原合成的抑制作用不明显(P>0.05)，当药物浓度上升为10～20mmol/L时，抑制作用增强，呈明显药物浓度依赖关系。结论 钙调蛋白拮抗剂抗肝纤维化的作用是抑制了贮脂细胞的增殖及胶原合成。

    

REGULATION OF CALMODULINANTAGONISTS ON PROLIFERATION AND COLLAGEN SYNTHESIS OF FAT STORING CELLS

Jing Xubin, Li Dingguo, LuHanming. Department of Gastroenterology, First Affiliated Hospital, Shantou UniversityMedical College, 515031

【Abstract】 Objective To investigate the antifibrotic mechanism ofcalmodulin antagonists(CaMA) on fat-storing cells(FSC) . Methods FSC wereisolated with method of density gradient centrifugation after digest in pronase E and collagenase, and then observed the effect of CaMA on proliferation and collagen synthesisof FSC, by means of MTT, the measuring incorporation of 3H-proline. Results The results showed that the suppresive effects of CaMA at low concentration was notsignificant, but was in a drug concentration-dependent manner at 10～20mmol/L. Conclusion The anti-fibrotic mechanismof CaMA is to inhibit proliferation and collagen synthesis of FSC.

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