郑书芳 柯杨 100034 北京医科大学临床肿瘤学院 北京市肿瘤防治研究所遗传室 中华肿瘤杂志 1999 0 21 6


    关键词：胃粘膜；细胞系；细胞核原位杂交；肿瘤抑制基因 期刊 zhzlzz 0 基础研究 fur --> /literature/library/imagebase/imagebase03.asp?keyword=胃粘膜；细胞系；细胞核原位杂交；肿瘤抑制基因


    

【摘要】 目的 在分子水平研究人正常胃粘膜上皮永生细胞株(GES-1)多种已知癌基因、抑癌基因的细胞内拷贝数，并对这一细胞的特性进行鉴定。方法 用载有APC、Rb、c-met基因的质粒经切口平移直接标记生物素制作探针，与GES-1间期细胞核原位杂交(FISH)。结果 APC基因双拷贝的核为48%，三拷贝的核占22%；Rb基因双拷贝核占71%；c-met基因双拷贝的核占80%。结论 GES-1细胞系在分子水平介于正常与恶性之间，仍可作为研究癌变过程分子机制的人类体外模型。

Study of APC, Rb,c-met gene copy numbers of human gastric mucosa epithelial cell line GES-1

ZHENG Shufang, KE Yang. Beijing Institute for Cancer Research, School ofOncology, Beijing Medical University, Beijing 100034

【Abstract】 Objective To Study the copynumber of oncogene/tumor suppressor genes in GES-1 cell line to identify itscharacteritics.Methods Bio-14-dATP was incorporated into APC, Rb, c-met gene cloned inplasmid by nick translation. Fluorescence in situ hybridization (FISH) of interphasenuclei of GES-1 cells was performed.Results Two copies of APC were shown in 48%interphase nuclei and 3 copies in 22%; 71% and 80% cells had normal copies of Rb and c-metgenes in their nuclei, respectively.Conclusion GES-1 cell line is a relatively normalgastric mucosa epithelial cell line and can be used as a human in vitro model system forthe study of carcinogenesis.

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