刘伦旭 覃扬 周清华 石应康 孙芝琳 孙泽芳 610041 成都，华西医科大学附属第一医院胸心外科(刘伦旭、周清华、石应康)，基础医学院生化教研室(覃扬、孙芝琳、孙泽芳) 中华肿瘤杂志 1998 0 0 5


    关键词：肺肿瘤 nm23基因 基因表达 印迹法，RNA 期刊 zhzlzz 0 基础研究 fur -->


    

【摘要】 目的 探讨nm23基因表达在人肺癌中的作用。方法 通过Northern印迹杂交，检测40例人肺癌组织和19例非癌肺组织的nm23-H1和nm23-H2 mRNA表达，采用地高辛标记和检测系统显示杂交信号，并分析mRNA表达与肺癌临床特征的关系。结果 低分化鳞癌的nm23-H2 mRNA表达较中高分化鳞癌显著降低(P<0.01)，小细胞肺癌的nm23-H1和nm23-H2 mRNA表达较肺鳞癌明显降低。但在有或无淋巴结转移的原发癌灶组织间，以及肺癌的临床分期间，nm23基因mRNA表达差异无显著性(P>0.05)。结论 nm23基因mRNA表达与肺癌的组织分化有关，未发现其在肺癌中的癌转移抑制作用。

    Northern blot analysis of nm23 gene expression in human lung cancer Liu Lunxu, Qin Yang, Zhou Qinghua, et al. Department of Thoracocardiac Surgery, The First University Hospital, West China University of Medical Sciences, Chengdu 610041

    【Abstract】 Objective To investigate the role of nm23 gene expression in lung cancer. Methods Forty human lung cancer tissues and 19 non-cancerous pulmonary tissues were studied for their nm23-H1 and nm23-H2 mRNA expression with nonradioactive Northern blot hybridization. Correlation of nm23 mRNA expression with clinical features of lung cancer was analyzed. Results The mRNA expression of nm23-H2 gene in poorly differentiated squamous-cell carcinoma was significantly decreased compared to that in moderately differentiated squamous-cell carcinoma. The mRNA expression of nm23-H1 and nm23-H2 genes in small cell lung cancer was decreased compared to that in squamous-cell carcinoma. No significant difference in nm23 mRNA expression was observed in lung cancers with and without lymph node metastasis, nor was there significant difference in patients with lung cancers in different stages. Conclusion The mRNA expression of nm23 gene is correlated with the degree of differentiation of lung cancer, but there is no evidence of metastasis suppression effect by nm23 gene.

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