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IL-2基因修饰人骨肉瘤细胞的制备及骨肉瘤患者IL-2和sIL-2R测定
http://www.100md.com 《中华骨科杂志》 1999年第1期
骨肉瘤|白细胞介素2|逆转录病毒科|基因疗法,关键词:
     郑强 范清宇 郭爱林 710038西安,第四军医大学唐都医院全军骨科中心 中华骨科杂志 1999 0 19 1


    关键词:骨肉瘤;白细胞介素2;逆转录病毒科;基因疗法 期刊 zhgkzz 0 骨肿瘤 fur -->


    

【摘要】目的 IL-2基因修饰人骨肉瘤细胞的制备及骨肉瘤患者IL-2、sIL-2R水平测定。方法 用逆转录病毒载体将人IL-2基因插入人骨肉瘤细胞系制备成IL-2基因修饰骨肉瘤细胞;用放射免疫法及ELISA法检测骨肉瘤患者血清内IL-2、sIL-2R水平。结果 经G418筛选和PCR鉴定后制备成功人骨肉瘤IL-2基因修饰细胞,并证实其有分泌IL-2功能,用MTT法测得其培养上清中IL-2表达量为每1×105 细胞24小时50~800u。骨肉瘤患者免疫功能调查发现其血清IL-2水平明显低于正常,而sIL-2R则明显高于正常,术后两者可逐渐正常。结论 制备成功IL-2基因修饰骨肉瘤细胞并发现骨肉瘤患者IL-2水平低下,为进行骨肉瘤基因治疗创造条件。

    The Preparation of Human Osteosarcoma Cells Modified by IL- 2 Gene and the Determination of IL- 2 and sIL- 2R in Patients with Osteosarcoma

ZHENG Qiang, FAN Qingyu, GUO Ailin.

    Orthpaedic Centre, Tangdu Hospital, Affiliated to The Fourth Military Medical University, Xi’an 710038

Abstract Objective To produce human osteosarcoma cells modified by IL- 2 gene and investigate the level of IL- 2 and sIL- 2R in patients with osteosarcoma. Methods The human IL- 2 gene was inserted into human osteosarcoma cells with retroviral vector and the tumor vaccine was produced. IL- 2 and sIL- 2R level in patients with osteosarcoma was determined with radioimmunoassay and ELISA. Results The human osteosarcoma cells modified by IL- 2 gene was produced successfully after G418 resistence selection and analysed by PCR; they could secrete IL- 2 in vitro. The production of IL- 2 was 50~ 800 u per 1× 10 5 cells every 24 h. The data about patients’immune state indicated that IL- 2 was lower than normal control and sIL- 2R higher. Conclusion The success in production of the human osteosarcoma cells modified by Il- 2 gene makes it possisle to carry out further reseach in gene therapy for human osteosarcoma.

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