孙抒 李龙山 孙东植 金昌范 133000 延吉市，延边大学医学院病理学教研室 中华肿瘤杂志 1999 0 21 6


    关键词：乳腺肿瘤；癌前病变；细胞凋亡；细胞增殖 期刊 zhzlzz 0 临床研究 fur -->


    

【摘要】 目的 通过原位观察乳腺癌及其癌前病变中细胞凋亡与细胞增殖的表达，探讨其在乳腺组织恶性转化进程中的作用。方法 利用原位DNA断裂点的3-羟基末端标记(TUNEL)技术、增殖细胞核抗原(PCNA)免疫组织化学染色原位观察31例乳腺癌、20例乳腺不典型增生、20例乳腺单纯性增生中的细胞凋亡与细胞增殖，以8例正常乳腺组织为对照。结果 正常乳腺组织中凋亡细胞多位于乳腺导管及腺泡的近腔面，增殖细胞位于基底部；乳腺单纯性增生、乳腺不典型增生及乳腺癌丧失了上述分布特征。乳腺不典型增生中凋亡细胞和增殖细胞密度均显著高于乳腺癌和正常乳腺组织(P<0.01)，乳腺癌中的凋亡细胞和增殖细胞密度也显著高于正常组织(P<0.01)。在乳腺单纯性增生和不典型增生中，凋亡细胞和增殖细胞密度比例分别为1∶1.3至1∶1.4，1∶1.1至1∶1.2；而在乳腺癌中其密度比例约为1∶2.3至1∶2.4。结论 细胞凋亡与细胞增殖异常可能是乳腺组织恶性转变过程中的重要指标之一。

In situ observationof apoptosis and proliferation in breast cancer and its precancerous lesions

SUN Shu, LI Longshan, SUN Dongzhi, et al. Department of Pathology,Yanbian University Medical College, Yanji 133000

【Abstract】 Objective To investigate theeffects of apoptosis and proliferation on malignant transformation of breast precancerouslesions by in situ observation.Methods The density and distribution ofapoptotic cells were examined in 31 cases with breast cancer, 20 with dysplasia and 20with hyperplasia, using TUNEL (TdT-mediated dUTP nick end labeling) technique andimmunohistochemical method respectively. Eight normal breast tissues were used as thecontrol.Results In normal breast tissue, apoptotic cells were locatedat the lacteals and gland follicles, facing the cavity. The proliferating cells werelocated at the basal layer of mucosa. These characteristics of distribution were lost inbreast cancer and precancerous lesions. The density of apoptotic cells and proliferatingcells of dysplasia was significantly higher than that in breast cancer and normal mucosa,The density of apoptotic cells and proliferating cells in breast cancer was significantlyhigher than that in normal mucosa. The ratio of apoptotic to proliferating cells inhyperplasia and dysplasia was 1∶1.3～1.4, 1∶1.1～1.2 respectively, and 1∶2.3～2.4in breast cancer.Conclusion Abnormality in cell apoptosis andproliferation may play important role in the genesis of breast cancer.

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