栾杰 蔡哲 杨佩瑛 宋业光 马海欢 唐勇 李艳姝 彭君 李丹 北京，中国医学科学院中国协和医科大学整形外科医院 100041 中华整形烧伤外科杂志 1999 0 15 3


    关键词：组织工程学；软骨细胞；脱细胞软骨基质；细胞培养 期刊 zhzxsswkzz 0 组织工程学 fur -->


    

【摘要】 目的 探讨脱细胞软骨基质对软骨细胞体外生长的影响。方法 以脱细胞异体兔耳软骨基质为支架材料，以脱细胞异体兔耳软骨膜为对照，体外种植兔耳软骨细胞进行常规培养，观察软骨细胞的生长状况和增殖情况。结果 软骨细胞进入脱细胞异体软骨基质构架裸露的空穴，生长旺盛。而在脱细胞异体软骨膜上，软骨细胞不易生长。结论 支架材料的表面性状对细胞生长有显著影响，异体脱细胞软骨基质可提供适宜于软骨细胞生长的良好环境，有可能发展成为软骨组织工程的一种天然支架材料。

Culture of chondrocytesusing allogenous acellular cartilaginous matrix

LUAN Jie, CAI Zhe, YANG Peiying,et al.Plastic Surgery Hospital, Chinese Academy of Medical Sciences, Beijing 100041

【 Abstract 】 Objective Toobserve the effect of chondrocyte culture with natural ECM made from allogenous acellularcartilage.Methods The allogenous acellular cartilaginous matrix was usedas a scaffold in chondrocyte cultrue in vitro, comparing with the acellular perichondrium.The growth and regeneration of the chondrocytes in vitro were observed. Results The chondrocytes seeded on the acellular cartilaginous matrix grew well while thechondrocytes on the acellular perichondrium matrix grew poorly.Conclusion The surface condition of the scaffold has a notable influence on the growth of the cells.The allogenous acellular cartilaginous matrix may provide a suitable condition for chondrocytes, and it has the possibility to be developed as a natural scaffold for tissueengineering.

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