关键词：断指；血管；冷藏；再植
【摘 要】 目的： 研究断指常温及冷藏于0℃～4℃时血管内皮细胞超微结构的变化，阐明冷藏对血管内皮细胞的保护作用，提出断指延迟再植的参考时限。方法： 将断指体分组冷藏后定时取指动脉标本电镜观察血管内皮细胞超微结构变化。结果： 室温组14小时后血管内皮细胞出现线粒体有空泡、核染色质凝集、核固缩等细胞坏死的早期变化；单纯冷藏组可放置50小时，用细胞内液泡浸后冷藏放置到64小时，才出现此坏死改变；加入能量合剂及氧自由基消除剂不能延长保存时限。结论： 室温25℃断指保存至14小时，单纯冷藏保存至50小时，用细胞内液泡浸后再冷藏至64小时时，血管内皮细胞均出现细胞坏死的早期变化，此时限可作为临床延迟再植断指的参考保存时限。
PROTECTION OF COLD STORAGE TO ENDOTHELIAL CELLS OF DIGITAL ARTERY Lin Ben dan，Zhong Zhigang， Qiu Xueli, et al.Department of Orthopaedics，Central Hospital，Shantou，Guangdong 515000
【ABSTRACT】 Objective ： To study the dynamic ultrastruture changes of endothelial cells of digital artery stored at room temperature and low temperature(0℃～4℃).To advance the reference time limit of delayed replantation of severed digit.Methods： Five digits of a patient suffered from destructive injury at wrist were used as the experimental materials.The digits were divided into four groups and stored at low temperature and room temperature.Digital artery was cut regularly and the ultrastructure changes of endothelial cells were observed under electron microscope.Results： The limited time that endothelial cells of digital artery appeared necrosis changes such as vacuole in mitochondrion ......
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