朱晓海 何清濂 林子豪 上海，第二军医大学长征医院整形外科 200003 中华整形烧伤外科杂志 1999 0 15 3


    关键词：前脂肪细胞；细胞培养；脂肪组织 期刊 zhzxsswkzz 0 论 著 fur -->


    

【摘要】 目的 更深入地研究人体脂肪组织增生和肥大的生物学特征。方法 选用成人的纯脂肪颗粒，采用“贴壁-天花板”培养法培养出梭形细胞。结果 培养出的梭形细胞成分均一，增殖旺盛，分化率高。经形态学动态变化、生长曲线、甘油磷酸脱氢酶活性变化、油红O脂肪染色及对胰岛素和地塞米松反应的测定，证明是功能活跃的前脂肪细胞，并在体外重现了其增殖、肥大的全过程。结论 在成熟的脂肪组织中存在着功能活跃的可调控组分。这是在国内首先建立的一种新的简便的培养人前脂肪细胞的方法，为进一步研究肥胖及探讨提高脂肪移植成活率奠定了扎实的基础。

Human preadipocyte cultureand the establishment of hyperplasia and

    hypertrophy model

ZHU Xiaohai,HE Qinglian,LIN Zihao.Department of Plastic and Reconstructive Surgery, Second Military MedicalUniversity,Shanghai 200003

【 Abstract 】 Objective Tobetter understand hyperplasia and hypertrophy properties of human adipose tissue.Methods Using an “ adhesion-ceiling” culture strategy,fibroblast-likecells from adult pure adipose granules were successfully cultured.Results The cells were highly homogeneous,highly proliferative and with a high differentiationrate.Their dynamic morphological changes,growth curve,glycerophosphate dehydrogenasepattern,oil red O staining,and reaction to insulin and dexamethasone all verified theirpreadipocyte identity.Under controlled conditions,the preadipocytes replayed their in vivohyperplasia and hypertrophy process.Conclusion In mature human adiposetissue there exist functionally active fractions that can be modified.This study laid thebasis for further probing into obesity and fat autotransplantation.

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