关键词：骨髓移植 巨细胞病毒感染 聚合酶链反应
【摘要 】 目的 为骨髓移植患者寻找较好的诊断和治疗巨细胞病毒(CMV)感染的分子生物学依据。方法 用聚合酶链反应(PCR) 方法测尿中CMV-DNA，逆转录(RT)-PCR法测血中CMV-即刻早期抗原(IE)mRNA。结果 在被测的27例患者103份标本中，发现10例CMV感染者的尿CMV-DNA及血IE mRNA几乎同时阳转。在连续使用抗病毒药甘昔洛瓦(DHPG)治疗的6例患者中，IE mRNA约一周左右阴转，而DNA约20天左右阴转。结论 CMV-IE mRNA可能反映病毒即刻早期转录的停止,对临床抗病毒治疗可能是一个较好的参考指标。
Clinical value of IE-mRNA detection in bone marrow transplantation patients with CMV infection
ZHANG Zheng, LI Li.
Department of Clinical Laboratory, 2nd Clinical Hospital, Beijing Medical University, Beijing 100044
【 Abstract 】 Objective To find out a specific marker for diagnosis and treatment of active human cytomegalovirus (CMV) infection in patients with bone marrow transplantation.Methods CMV-DNA in urines was detected by PCR, and CMV-IE mRNA in blood by RT-PCR.Results 103 samples from 27 patients were detected by molecular biology method for CMV. The positive results were got hardly at the same time from CMV DNA and CMV-IE mRNA in 10 patients with CMV infection. In 6 patients treated with DHPG, CMV-IE mRNA first (about one week) become negative, and CMV-DNA turned to be negative on 20 days from the initial treatment of DHPG.Conclusion CMV-IE mRNA may reflect the cessation of virus immediate early transcription, and may be a promising reference value in anti-CMV treatment.
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