王志军 倪语星 200025上海第二医科大学瑞金医院检验科 中华医学检验杂志 1999 0 22 6


    关键词：β-内酰胺酶 聚合酶链反应 寡核苷酸探针 期刊 zhjyyxzz 0 论著摘要 fur -->


    

【摘要 】 目的 建立有效和实用的方法，检测质粒介导的TEM型超广谱β-内酰胺酶并对其分型。方法 采用聚合酶链反应-序列特异性寡核苷酸探针杂交(PCR-SSO)技术，首先用TEM β-内酰胺酶基因的通用引物进行扩增，再用序列特异性寡核苷酸探针与扩增产物进行杂交，对多株标准产酶菌株进行分型。结果 产TEM β-内酰胺酶的菌株扩增阳性，而产其他β-内酰胺酶的菌株扩增阴性，探针杂交的结果符合标准菌株的产酶型别。结论 PCR-SSO是一种有效的检测TEM超广谱β-内酰胺酶及分型的方法。为临床检验、流行病学调查和研究提供了依据。

Detection and typing of plasmid-mediated TEM extended spectrum β -lactamase

     WANG Zhijun, NI Yuxing.

    Department of Clinical Laboratory, Ruijin Hospital, Shanghai 200025

【 Abstract 】 Objective To establish an effective method for the detection and typing of TEM extended spectrum β -lactamase(ESBL). Methods The polymerase chain reaction-sequence specific oligonucleotide (PCR -SSO) was used. PCR was performed with β -lactamase common primers. Then the PCR production was hybridized with digoxigenin-labeled sequence specific oligonucleotide. Results Strains which produce TEM β -lactamase were positive by PCR, whereas others were negative. The oligotypings which determined by hybridization accorded with their real kinds. Conclusion The PCR-SSO is an effective method for the detection and typing of TEM-ESBL, can be used in clinical detection and epidemiological study .

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