【摘要】 目的 研究静脉插管感染病原菌分离及耐药状况。方法 静脉管端标本培养及血培养使用全自动血培养仪；微生物鉴定及药敏主要使用全自动微生物鉴定系统；分析患者各种微生物分离率与免疫状况及患者病区分布的关系。结果 72小时内100份标本全部得到阳性结果，24小时分离率69.5%革兰阳性球菌与革兰阴性杆菌总分离率相等。血液科杆菌感染率63.2%高于球菌18.4%，肿瘤外科球菌感染率66.7%高于杆菌18.5%。在球菌感染中耐甲氧苯西林金黄色葡萄球菌占90.9%，耐甲氧苯西林凝固酶阴性葡萄球菌70.0%。免疫低下组真菌感染率16.9%高于免疫正常组2.9%。革兰阳性球菌、革兰阴性杆菌耐药率均较国内最近报道为高。结论 静脉插管院内感染病原菌的种类与免疫状况有关；全部病原菌耐药情况严重。

    Analysis of intravenous catheter infection in 100cases Zhang Zheng, Wang He, Zhao Surui, et al.Department of Clinical Laboratory, People′sHospital, Beijing Medical University, Beijing 100044

    【Abstract】 Objective To identifybacteria from infections caused by intravenous catheter and its resistance to antibiotics.Methods The bacteria from interavenous cacheters andblood were cultured with BACT ALTER 120. The bacteria identified and susceptibility wasmade with VITEK AMS 60. A relationship was studied between the bacteria isolated rate andimmune state. Results The positive results of 100samples were obtained within 72 hours. The isolation rate in 24 hours was 69.50%. Thetotal isolation rate was equal between G+ cocci and G- bacilli. In hematology departmentthe infection rate of bacilli (63.2%) was higher than that of cocci (18.4%). In tumorsurgery the infection rate of cocci (66.6%) was higher than that of bacilli (18.5%). TheMRSA was 91.0% in staphylocci infection and MRCNS 77.8% in others G⁺ cocci.Inthe immunnocompromised group, the infection rate of fungi was 16.9% which was higher thanin other group (2.9%). The results of susceptibility showed higher resistance toantibiotics of G^- and G⁺ bacteria than that reported in China.Conclusions The kinds of bacteria were relatedimmune state of patients. There was a serious resistance to antibiotics in all isolated becteria.

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