白晋 伍烽 王智彪 阮祥燕 杜永洪 孔繁斌 王芷龙 陈迅 童艺 齐家俊 刘川 胡凯 邹建中 王文见 彭兴礼 陈文直 王志刚 400046 重庆医科大学医学超声工程研究所 中华超声影像学杂志 1999 0 8 4


    关键词：超声疗法；高强度聚焦超声；肝；剂量效应关系；辐射 期刊 zhcsyxxzz 0 实验研究 fur -->


    

【摘要】 目的 探讨高强度聚焦超声(HIFU)非侵入性切除肝脏组织的剂量-效应关系及机体反应，旨在为体内深部组织肿瘤的无创性治疗提供实验基础。方法 采用重庆医科大学医学超声工程研究所自行设计制造的GL-1型治疗样机(1.6MHz,13153 W/cm² ,3 s)，对28只小型香猪肝脏组织进行定位损伤。于处理前1天、处理后1天、7天、14天、21天、28天测定体温、体重，抽血查肝肾功能。HIFU后3天解剖，对肝损伤区进行体积测定和病理学检查。结果 在40mm 深度范围内，HIFU可体外有效地定位损伤肝脏组织。辐照时间与肝组织凝固坏死体积的关系为(12.5±5.9)s/cm³ 。机体反应： 体温在处理后1天体温升高2.29℃，2周内体温升高1℃，2周后恢复正常。体重治疗前后无明显变化。处理前后肝肾功能无明显异常(P>0.05)。副作用：皮肤损伤4例(14.28%)，3～7天愈合； 胃损伤2例(7.14%)，禁食2天，自然进食，生存良好。结论 应用高强度聚焦超声非侵入性破坏香猪肝脏组织是有效的，此技术对非侵入性局部破坏人体实体瘤将有重要的临床应用前景。

Localized lesion to normalminiswine liver with high intensity focused ultrasound and the dose-effect relation

BAI Jin,WU Feng,WANG Zhibiao,etal.Institute of Ultrasonic Engineering in Medicine of Chongqing MedicalUniversity,Chongqing 400016

【Abstract】 Objective Toexplore the dose-effect relation and organic reaction in the case of in vivo ablation ofliver tissues with high intensity focused ultrasound (HIFU) so as to prepare HIFUsonication as a noninvasive in vivo therapy for tumors located in deep-seated tissues.Methods The GL-1 therapeutic apparatus (1.6 MHz,13153 W/cm² ,and 3 s duration)developed by the Institute of Ultrasonic Engineering in Medicine of Chongqing MedicalUniversity was separately used to selectively destroy the liver tissues of 28miniswine.Measurement of temperature,body weight,and blood test of hepatic and renalfunctions were made the day before and after the sonication ,and on the 7th,14th,21st,and28th day following the ablation.After 3 days,the autopsy was applied and then the lesionswere measured and pathologically examined.Results It was observed thatHIFU is capable of bringing selective damage to tissues at depth of 40 mm and that theratio of sonication duration to the volume of coagulative necrosis was (12.5±5.9)s/cm³ .Thetemperature was 2.29℃ above normal one day after the sonication,1℃ above normal withintwo weeks after the sonication,and back to normal again after two weeks.No obvious changesin body weight were observed before and after the treatment.No obvious anomaly of hepaticand renal functions were detected before and after the sonication(P>0.05).Side effects:skin burn 14.28%(4/28),it was recovered within 3-7 days; Gastric impairment7.14%(2/28),the pigs were not given food for 2 days,they recovered very well.Conclusions It is effective and safe to obtain in vivo ablation of tumors on large animalssuch as miniswine using high intensity focused ultrasound.This suggests the brightprospect of the clinical use of HIFU in the ablation of human tumors.

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