任晓燕 张玉敏 曲忠森 葛宝林 中华神经科杂志 1998 0 31 4


    关键词：脑缺血；；再灌注损伤；；谷氨酸；；牛磺酸 期刊 zhsjkzz 0 *论著* fur -->


    

【摘要】 目的 探讨牛磺酸对鼠脑缺血再灌注(IR)时皮层、海马、纹状体谷氨酸转运体功能的影响。方法 采用3血管夹闭、松夹制作鼠IR模型。利用脑组织突触膜颗粒对3-H-L-谷氨酸摄入量的测定及分光光度法观察谷氨酸转运体、丙二醛(MDA)含量及超氧化物歧化酶(SOD)活性的变化。结果 与对照组相比，IR组皮层、海马及纹状体谷氨酸转运体的功能及SOD活性低(P<0.05,0.01)，MDA含量高(P<0.05,0.01)。与IR组相比，牛磺酸组鼠脑皮层、海马及纹状体的功能及SOD活性高(P<0.05，0.01)，MDA含量低(P<0.05,P<0.01)。结论 牛磺酸可改善IR后皮层、海马及纹状体谷氨酸转运体的功能，其机制可能与清除自由基有关。

    Influence of taurine on the function of glutamate transporterin rats with injury of cerebral ischemia-reperfusion Ren Xiaoyan,Zhang Yumin, Qu Zhongsen， et al. Department of Pathophysiology, Qingdao Medical College,Qingdao 266021

    【Abstract】 Objective To investigate theeffect of taurine on the function of glutamate transporter in rats with injury of cerebral ischemia-reperfusion. Methods A model of ischemia-reperfusion injury (IR) was performed by clipping three arteries and then byreleasing them to reperfuse blood into the ischemic brain in rats. Glutamate transporterfunctions were studied by means of assay of 3-H-L-glutamate uptake in synaptosomes, andMDA contents and SOD activities in cerebral cortex, hippocampus, striatum byspectrophotometry.Results Glutamate transporterfunction and SOD activity in IR group were significantly decreased (P<0.05,0.01); content of MDA were significantly increased (P<0.05, 0.01) while compared with control group. Glutamatetransporter function and SOD activity in taurine group were significantly increased(P<0.05, 0.01); MDA content was significantly decreased (P<0.05, 0.01) while compared with IR group. Conclusion Taurine can improve glutamate transporter function in IR rats. Themechanism might be related to eliminate free radicals.

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