施焕中 覃寿明 肖常青 陈一强 许辉 530021 南宁，广西医科大学第一附属医院呼吸内科 中华结核和呼吸感染 1999 0 22 12


    关键词：嗜酸细胞；淋巴细胞；气道高反应性；哮喘 期刊 zhjhhhxgr 0 论著 fur -->


    

【摘要】 目的 探讨CD86分子对抗原引起气道炎症和气道高反应性的影响，加深认识CD86在支气管哮喘发病机制中的作用。方法 应用鸡卵清蛋白致敏和刺激BALB/c小鼠(每组8只)以诱导嗜酸细胞(EOS)聚集到气道，收集支气管肺泡灌洗液(BALF)细胞并以流式细胞仪检测CD86分子的表达水平；观察静脉注射抗CD86单克隆抗体后BALF中EOS数和气道反应性的变化。结果 小鼠经抗原致敏和刺激后BALF中可以见到大量的EOS，气道反应性亦明显升高，BALF细胞所表达的CD86水平也随之增高。经抗CD86单克隆抗体处理后，BALF中EOS 数降低了67%(P<0.01)；同时，气道反应性也下降69%(P<0.01)。此外，抗CD86单克隆抗体还可以抑制肺组织局部白细胞介素4和白细胞介素5的产生。结论 抗CD86单克隆抗体能够抑制气道EOS浸润和降低气道反应性，其作用机制可能是通过抑制局部白细胞介素4和白细胞介素5产生而实现。提示抑制气道抗原呈递细胞的活性应有益于哮喘的治疗。

    

A study on the roles of CD86 in antigen-induced eosinophil infiltration into airways and airway hyperresponsiveness

SHI Huanzhong, QIN Shouming, XIAO Changqing, et al .

    Department of Respiratory Medicine, First Affiliated Hospital, Guangxi Medical University, Nanning 530021

【 Abstract】 Objective To explore the effect of CD86 on antigen-induced eosinophil infiltration into the airways and airway hyperresponsiveness in sensitized mice, and further elucidate the role of CD86 in the pathogenesis of bronchial asthma. Methods Female BALB/c mice (n=8 for each group) were sensitized and challenged with ovalbumin to induce airway eosinophilia and airway hyperresponsiveness . Airway responsiveness was espressed by the provocative concentration of acetylcholine causing 50% increase in respiratory resistance (PC₅₀ ).Effect of anti-CD86 monoclonal antibody (mAb) on antigen-induced changes of eosinophil numbers in brochoalveolar lavage fluid (BALF) and airway reactivity was observed. CD86 expression on BALF cells was detected by flow cytometry and concentrations of interleukin-4 and interleukin-5 in homogenized supernatant of lung tissue were determined by ELISA. Results In sensitized mice challenged with ovalbumin 20 minutes once a day for 6 days , the number of BALF eosinophils was (9.2±l.5)×10⁸ /L. However, no eosinophil could be found in the BALF from mice without ovalbumin sensitization and challenge. Also, ovalbumin treatment led to PC₅₀ value decrease from (0.66±0.13) g/L to (0.17±0.07) g/L (P<0.01). CD86 expression on BALF cells from ovalbumin sensitized- and challenged-mice (36.4±6.2) was much higher than that from control mice (12.3±3.6, P<0.01). In mice treated with intravenous injection of anti-CD86 mAb before each challenge, BALF eosinophils decreased by 67% (P<0.01 ), and PC₅₀ value increased by 69% (P<0.01). Our results showed that anti-CD86 mAb prevented antigen-induced airway eosinophilia and airway hyperresponsiveness accompanied by a decrement of levels of both interleukin-4 and interleukin-5 in lung tissue. Conclusions Anti-CD86 mAb is able to inhibit antigen-induced airway eosinophilia and to ameliorate airway hyperresponsiveness, possibly by inhibiting production of interleukin-4 and interleukin-5. These data suggested that the blockade of airway antigen-presenting cells′ funct ions couid be of value in treatment of human asthma.

     【 Key words】 Eosinophil Lymphocyte Airway hyperresponsiveness Asthma

支气管哮喘最主要的病理特征是T淋巴细胞和嗜酸细胞(EOS)浸润到气道粘膜组织 ......