劳杰 熊良俭 顾玉东 黄慧思 200040 上海医科大学附属华山医院手外科(劳杰、顾玉东)；香港威尔逊亲王医院骨科(熊良俭、黄慧思) 中华手外科杂志 1999 0 15 2


    关键词：神经再生；许旺氏细胞；细胞；培养的；；免疫酶技术；大鼠；Spraque-；Dawley 期刊 zhswkzz 0 基础研究 fur -->


    

【摘要】 目的 应用双酶消化法来提取高纯度和高数量的周围神经雪旺细胞。方法 对双酶消化周围神经的时间由30分钟延长至80～ 90分钟；机械分离周围神经束的方法改为在放大16倍手术显微镜下分离神经束。对培养细胞进行雪旺细胞数量、Lowry蛋白含量的测定;并用同位素标记测定细胞量和免疫组织化学法观察。结果 从成年SD大鼠1 cm长一段上臂尺神经中,用该法可提取1.72 × 10⁷ 个雪旺细胞 / 毫升,雪旺细胞的纯度和数量是以往所有提取方法中最高的，并且这些雪旺细胞的细胞形态和再生功能均正常。结论 该改良方法为研究周围神经再生的机理,提供了一个重要的手段。

ModifiedSchwann cell culture from adult SD rats

LAO Jie, HungLK, GU Yudong, et al. Department of Hand Surgery,HuashanHospital,Shanghai Medical University, Shanghai 200040

【Abstract】 Objective Tointroduce a new technique of controlled enzyme digestion of peripheralnerves to obtain a highly purified suspension of Schwann cells. Methods The peripheral nervespecimen was digested by two enzymes for 80 to 90 minutes. The nervefascicle were then extracted under 16 × microscope instead of nakedeyes. The number of Schwann cells was counted after isotopiclabeling.The content of L - protein was examined by S - 100 proteinstaining. Results Anaverage of 1.72 ×10⁷ cells/ml was obtained from 1 cmsegment of the ulnar nerve in the grown - up SD rat. The cells werehighly purified, with normal morphology and function. Conclusions Thismodified method for extraction and culture of Schwann cells provides anew means for the study of the mechanism of peripheral nerveregeneration.

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