孙爱华 梁振福 李津婴 李兆基 胡正元 周义德 张锦方 叶煦亭 孙爱华 200003 上海市，第二军医大学长征医院耳鼻咽喉科；李兆基、周义德、张锦方 第二军医大学长海医院耳鼻咽喉科，李津婴 血液科，叶煦亭 电镜室；梁振福、胡正元 海军医学研究所 中华航海医学杂志 1999 0 0 1


    关键词：铁；稳态噪声；听损伤；静纤毛 期刊 zhhhyxzz 0 航海环境医学 fur -->


    

【摘要】 目的 探讨应用强化铁营养对抗稳态噪声致聋作用的可能性。方法 40只健康幼龄Wistar大鼠按随机化原则分为两组，每组20只：A组饲标准饲料；B组饲强铁化饲料。将A、B两组大鼠同时暴露于110dB SPL 20～10 000 Hz稳态白噪声，持续40分钟。根据暴露前后听性脑干反应(ABR)阈差值判定听力阈移，扫描电镜及光镜下观察耳蜗显微结构和听毛细胞表面超微结构变化。结果 暴露后2小时、3天和11天的B组听力阈移值显著小于A组；暴露后21天，虽然两组听力阈移均数值的差异无显著意义，但A组ABR阈值均数仍显著大于该组大鼠噪声暴露前平均听阈。扫描电镜下观察，暴露后2小时的A组大鼠外毛细胞静纤毛出现严重扭曲、倒伏、排列紊乱等病理变化；3天后，上述病变明显减轻；21天后，部分听毛细胞静纤毛排列紊乱仍未能完全恢复。在B组大鼠，外毛细胞静纤毛暴露2小时后有轻度扭曲及排列紊乱，3天后这些病变基本恢复，21天后外毛细胞静纤毛形态完全恢复正常。结论 强化铁营养具有对抗稳态噪声听损伤作用，其机理可能与其对含铁酶类活性的增强作用有关。

    

    An experimental study of the effects of fortifiediron nutrition on hearing loss induced by steady-state white noise SUN Aihua, LIANG Zhenfu, LI Jinying, et al. Department of Otolaryngology, Changzheng Hospital, Second Military Medical University, Shanghai 200433

     【Abstract】 Objective 【Abstract】 Objective To study the effects of fortified iron nutrition on hearing loss induced by steady-state white noise.Methods 40 Wistar rats wererandomized into two equal groups(A and B, each 20 animals). The group A rats were fed withnormal dietary iron and the group B rats were fed with fortified iron nutrition diet. Theywere simultaneously exposed to a steady-state white noise (20～10 000 Hz) at 110 dB SPL for 40 min in a sound-proof room. Hearing loss was determined by comparing the thresholds of auditory brainstem response(ABR) observed in the same animal before and after the noise exposure. The cochlear morphologic changes were examined with optic microscopy and scanning electron microscopy.Results The ABR threshold shifts at 2 h, 3 d and 11 d after the noise exposure in the group B rats were significantly smaller than those in the group A rats. No permanent threshold shift was seen in any of the group B rats. Whereas in the group A rats, the mean value of ABR threshold at 21 d after exposure was significantly higher than that before exposure. The pathologic changes of the outer hair cell stereocilia at 2 h after exposure were much less severe in group B than that in group A. And at 21 d after noise exposure, the stereocilia appeared perfectly normal and well defined in group B, where as there were still remained some abnormalities of stereocilia in group A.Conclusions Adequate and well designed fortified iron nutrition diet has the effect of preventing noise-induced hearing loss, and the action mechanism may be related with its enhancing effect on the activity of iron containing enzymes.

     【Key words】 【Key words】 Iron Noise,steady-state Hearing loss Stereocilia

(Chin J Naut Med, 1999,6:13)

稳态噪声是导致各类舰船、飞行人员以及某些产业工人发生噪声性听力损伤的主要原因之一 ......