陈纪宁 屠重棋 龚 全 胡云洲 华西医科大学附属第一医院骨科(成都，610041)；陈纪宁 现在重庆三峡中心医院骨科(重庆万州区，404000) 中国修复重建外科杂志 1999 0 13 5


    关键词：酸性成纤维细胞生长因子；引导性骨再生；骨缺损 期刊 zgxfcjwkzz 0 生长因子的研究与应用 fur -->


    

【摘 要】 目的 研究酸性成纤维细胞生长因子(αFGF)对引导性骨再生(GBR)的作用，增强GBR修复骨缺损的能力。方法 16只新西兰白兔分为四组，每组4只，造成兔双侧桡骨干10mm节段性骨缺损，以硅胶管桥接骨缺损，实验侧管内置入人基因重组酸性成纤维细胞生长因子(hraFGF)24μg，对侧管内注入生理盐水作对照。于术后2、4、6及8周各处死一组兔，作X线、大体、组织学观察。结果 实验侧术后2周即在骨断端髓腔、骨内膜及皮质断面处有新骨形成，并长入管内血肿，术后4周新骨长入血肿中心，8周完全骨愈合。对照侧在各阶段新骨形成均不如实验侧，8周时仅出现部分骨愈合。结论 酸性成纤维细胞生长因子(aFGF)可促进GBR，增强其修复骨缺损的能力。

EXPERIMENTAL STUDY ONSTIMULATION OF GUIDED BONE REGENERATION BY ACID FIBROBLAST GROWTH FACTOR

CHEN Ji-ning, TU Chong-qi,GONGQuan, et al.

Department of Orthopedic Surgery,First University Hospital,West China University of Medical Sciences. ChengduSichuan,P.R.China 610041

【 Abstract】 Objective To investigate the effect of acid fibroblast growth factor (aFGF) on guided bone regeneration (GBR), to study whether aFGF can promote the repairingability of GBR in bone defect.Methods 10 mm long segmental defects were created in the diaphysesof both radii in 16 New Zealand rabbits. The defect was bridged with a silicon tube. Humanrecombinant aFGF was instilled into the tube on the experimental side, while thecontralateral tube was instilled with saline as control group.The radiographic, gross andhistologic examination of the samples were analyzed at 2,4,6 and 8 weeks after operation.Results On the experimental side, therewas new bone formation in the bone medullary cavity,the endosteum and the section surfaceof the cortex at 2 weeks. At 4 weeks, at the center of the blood clot in the tube therewas new bone formation and bone defect was completely healed at 8 weeks. On the controlside,new bone formation was less in every period compared with that of the experimentalside. At 8 weeks, there was only partial healing of the bone defect.Conclusion It can be concluded that aFGF canpromote new bone formation and facilitate GBR in bone defect.

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