刘文革 罗永湘 同济医科大学附属同济医院骨科(武汉，430030) 中国修复重建外科杂志 1999 0 13 5


    关键词：脊髓损伤；成纤维细胞生长因子；继发性损害 期刊 zgxfcjwkzz 0 生长因子的研究与应用 fur -->


    

【摘 要】 目的 探讨碱性成纤维细胞生长因子(bFGF)对脊髓损伤的早期保护作用。方法 选34只SD大鼠，随机分为正常组、对照组和治疗组，采用Allen氏技术，以10g×2.5 cm致伤力造成大鼠T₈ 急性脊髓损伤，并于损伤平面以下蛛网膜下腔置入细塑料管，治疗组给予bFGF治疗，对照组同时注入生理盐水。术后切取损伤区脊髓组织进行形态学观察和生化指标测定。结果 受损伤段脊髓组织水含量增多，钙离子水平升高，镁离子水平下降，白质内髓鞘结构紊乱，囊性变严重，而bFGF可明显改变上述变化。结论 bFGF对脊髓损伤早期的继发性损害有保护作用。

THE EARLY PROTECTIVEEFFECTS OF BASIC FIBROBLAST GROWTH FACTOR ON ACUTE SPINAL CORD INJURY IN RATS

LIU Wen-ge, LUO Yong-xiang.

Department of Orthopedic Surgery,Tongji Hospital, Tongji Medical University. Wuhan Hubei, P.R.China 430030

【 Abstract】 Objective To study the early protective effects of basic fibroblastgrowth factor(bFGF) on the experimental acute spinal cord injury.Methods Thirty-four SD rats were randomlydivided into three groups, and were subjected to contusion of thoracolumber spinal cord. Athin plastic tube was placed in subarachnoid space below the injury level for perfusion.The bFGF-treated rats were received 20 μ l bFGF(containing bFGF 100 U) at once,30 min,1,2,3,4,6,12,24 and 48hours after injury, and an equal volume of normal saline was given to the control group atthe same time. The injuried spinal cord was detected by morphological observation andbiochemical index after injury.Results The degree of ionic disorder in bFGF-treated rats wassignificantly ameliorated and the contents of H₂ O were also markedly decreased.The morphological finding showed that the damages of gray and white matter in bFGF-treatedrats were slighter than those of saline-treated rats. Conclusion bFGF has some protective effectson the secondary lesion of early spinal cord injury in rats.

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