洪 岸 李校坤 付忠国 徐 林 林 剑 洪 岸 李校坤 林 剑 暨南大学生物工程研究所(广州，510632)；付忠国 徐 林 北京医科大学人民医院骨科 中国修复重建外科杂志 1999 0 13 5


    关键词：碱性成纤维细胞生长因子；坐骨神经损伤；修复 期刊 zgxfcjwkzz 0 生长因子的研究与应用 fur -->


    

【摘 要】 目的 研究碱性成纤维细胞生长因子(bFGF)对周围神经损伤的促修复作用。方法 建立大鼠坐骨神经离断模型，镜下进行神经外膜缝合，局部滴加药物和术后肌注bFGF以及临床用药神经生长因子(NGF)，通过神经传导速度和功能指数评定观察坐骨神经修复情况。结果 术后1个月，以正常对照为标准，0基线为正常水平，生理盐水、NGF、bFGF使用组神经功能指数评定结果分别为：-114.30±10.34、-70.50±11.01、-50.45±7.82；术后3个月，分别为：-54.96±16.46、-35.21±10.80、-27.53±11.23，bFGF组神经传导速度明显快于生理盐水组和NGF组(P<0.01)。结论 bFGF对坐骨神经损伤具有显著的促功能修复作用，并且在神经损伤早期就能最有效地发挥作用，效果明显优于临床用药NGF。

EFFECT OF BASIC FIBROBLASTGROWTH FACTOR ON REPAIRING TRANSECTED SCIATIC NERVE IN RATS

HONG An, LI Xiao-kun, FUZhong-guo, et al.

Bio-engineering Institute,JiNanUniversity. Guangzhou Guangdong, P.R.China 510632

【 Abstract】 Objective To investigate the effects of basic fibroblast growth factor(bFGF) on repairing transected sciatic nerves in rats.Methods The animal models of thetransected sciatic nerve of 40 SD rats were established, which divided into 4groups:normal saline (NS) group, nerve growth factor (NGF) group,bFGF group and normalcontrol group. The epineurium of the transected sciate nerve was sutured under microscope,then bFGF or NGF was dropped into local sites and injected intramuscularly once a day for30 days after operation. Functional repair for the transected sciatic nerves was studiedby nerve conductive velocity (NCV) and sciatic nerve function index (SFI).Results As a criterion, the level of thenormal control group was regarded as zero, SFI of NS group, NGF group and bFGF group were-114.30±10.34,-70.50±11.01,-50.45±7.82 respectively at 1 month after operation, andthey were -54.96±16.46, -35.21±10.80, -27.53±11.23 respectively in 3 months afteroperation. NCV of bFGF group was significantly faster than NS group and NGF group.Conclusion bFGF can significantly promotethe functional repair of injuried peripheral nerve, and its effects are better than NGF.

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