傅小兵 孙同柱 王亚平 杨银辉 蒋礼先 顾小曼 常国友 盛志勇 解放军第304医院(北京，100037) 中国修复重建外科杂志 1999 0 13 5


    关键词：创伤; 创伤修复; 表皮细胞生长因子;碱性成纤维细胞生长因子 期刊 zgxfcjwkzz 0 生长因子的研究与应用 fur -->


    

【摘 要】 目的 采用小型猪背部创伤模型，定量研究与比较重组人表皮细胞生长因子(rhEGF)与重组人碱性成纤维细胞生长因子(rhFGF)的促修复效应与作用机制。方法 以创伤模型制作器切割小型猪背部皮肤，造成典型全层皮肤损伤。将16只小型猪背部的160个创面分成两大组，分别以三种不同剂量的rhEGF(50、10及0.5μg/创面)和rhFGF(150、90及30 U/cm² 创面)治疗，每组同时各设溶媒为阴性对照。以伤腔容积与创面愈合面积，新生毛细血管计数以及组织病理学方法评价修复效果。结果 经rhEGF与rhFGF治疗的创面组织修复速度均较同组阴性对照明显加快，表现为肉芽组织生成增多，再上皮化明显以及愈合时间缩短等。定量比较rhEGF与rhFGF的促修复效果，发现rhEGF的促再上皮化作用明显，而rhFGF对肉芽组织生长有特殊作用，两种生长因子均在中剂量时促修复作用最强。结论 rhEGF和rhFGF促修复效果的差异主要取决于它们不同的生物学特征以及作用机制。组织缺损较大，需大量肉芽填充的创面以应用rhFGF为好，而以再上皮化修复为主的创面则以选择rhEGF为佳。

COMPARATIVE STUDY OFEPIDERMAL GROWTH FACTOR AND BASIC FIBROBLAST GROWTH FACTOR ON WOUND HEALING

FU Xiao-bing, SUN Tong-zhu, WANGYa-ping, et al.

The 304th Hospital of PLA.Beijing, P.R.China 100037

【 Abstract】 Objective The biological effects of recombinant human epidermalgrowth factor (rhEGF) and recombinant human fibroblast growth factor (rhFGF) wereevaluated on the model of incised wounds in mini pigs.Methods Total of 160 incised wounds in 16mini pigs were divided into two groups (rhEGF group and rhFGF group), each containing 80wounds. In rhEGF group, 60 incised wounds were treated with different dosages of rhEGF(50,10 and 0.5 μ g/wound),and another 20 wounds were treated with solvent as control group. In rhFGF group, allwounds were treated in the same way as described in rhEGF group, the dosages of rhFGF were150,90 and 30 U/cm² respectively. The measurements of cavity volume and area inwound, histological examination were used to evaluate the results of wound healing. Results The results showed that woundhealing was accelerated in all wounds treated with rhEGF and rhFGF. In rhEGF group, thevelocity of re-epithelialization was faster than that of rhFGF group, however, newgranulation tissue in rhFGF was more than that of rhEGF group. Conclusion The results indicate that rhEGFand rhFGF can stimulate wound healing, however, the mechanisms and the biological effectsinvolved in these processes are quite different. It suggests that it is better to userhFGF in those wounds which need more granulation tissue formation and use rhEGF in thewounds which mainly need re-epithelialization.

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