曹 蕾 Felicia Rosenthal 焦向阳 Esther Tanczos Roland Mertelsmann

    G.Bjorn Stark 刘新垣 Peter Kulmburg 焦向阳 Esther Tanczos G.Bjorn Stark Department of Plastic Surgery, Freiburg University Medical Center, Germany;焦向阳 第二军医大学附属长海医院整形外科 中国修复重建外科杂志 1999 0 13 5


    关键词：基因治疗；创面愈合；成纤维细胞；表皮细胞生长因子 期刊 zgxfcjwkzz 0 生长因子的研究与应用 fur -->/info/meeting/meeting02.asp?keyword=整形外科学


    

【摘 要】 目的 探索通过转基因成纤维细胞向创面持续投递表皮细胞生长因子(EGF)促进创面愈合的新方法。方法 构建新型分泌型人EGF cDNA并转染人成纤维细胞株KMST6。将经照射的转基因细胞移植于裸鼠全厚创面。结果 稳定转染的细胞克隆可分泌有活性的EGF。移植后可在伤口组织中检测到人EGF，其含量缓慢下降，但至少可持续7天。结论 一次性移植少量转基因细胞可在创面愈合早期这一关键阶段持续向创面释放EGF。

CONTINUOUS DELIVERY OF EPIDERMAL GROWTH FACTOR TO WOUNDS IN VIVO BY GENETICALLY MODIFIED FIBROBLASTS TRANSFECTED WITH A NOVEL CHIMERIC CONSTRUCT

CAO Lei, Felicia Rosenthal, JIAO Xiang-yang, et al.

Shanghai Institute of Biochemistry, the Chinese Academy of Sciences. Shanghai, P.R. China 200031

【 Abstract】 Objective This paper aims to explore the new method of continuous delivery of epidermal growth factor to wounds by transfected fibroblasts to promote wound repair.Methods It was constructed a novel chimeric expression plasmid in which the biologically active portion of the human epidermal growth factor (EGF) gene was fused in-frame to the human granulocyte colony-stimulating factor signal sequence.Results Clonally selected human fibroblasts transfected with this construct could secrete biologically active EGF. After the transplantation of irradiated gene-transfected fibroblasts suspended in fibrin glue to murine full-thickness wounds, EGF could be demonstrated for at least seven days in the wounds, slowly decreasing from initially 470 ng/L to 140 ng/L in 7 days. No EGF was found in the wound at 14 days. Conclusion A single application of irradiated EGF gene transfected fibroblasts to wounds can continuously deliver the transgene in vivo and can be used to administer drugs to the wound bed during the crucial first seven days of wound-healing.

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