关键词:P16基因;胶质瘤;免疫化学结构;聚合酶链式反应-单链构象多态性
摘 要 目的 检测20例小儿脑胶质瘤中P16基因及其蛋白的存在情况。方法用聚合酶链式反应-单链构象多态性分析和免疫组织化学技术检测了20例1~14岁小儿脑胶质瘤。结果显示P16基因和P16蛋白的丢失率分别为50%(1020)及(520)。结论本文结果提示P16基因和蛋白缺失可能与部分小儿脑胶质瘤发生、发展有关。
Expression of p16 gene inpediatric gkiomas
MA Jinguang LI Nengde RANDesen.
Department of NeurosurgeryHuaDu Hospitak Huadu 527300
Abstract objective To detectthe exiting situation of p16 geneand protein in 20 cases ofpediatric gkiomas. Method 20cases of brain gkioma weredeteted by PCR- SSCR andimmunohistochemistric methods.Resukt The positive rate forkoss of p16 gene and protein were50%1020 and 25%520respectiveky. Conckusion Itwas indirectky suggested thatkoss p16 gene and proteinexpression might account for thepathogenesis and devekopment ofsome of pediatric gkiomas.
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