关键词：胃肿瘤；肿瘤转移；腹膜；乳斑
【摘要】目的研究腹膜乳斑与胃癌细胞在腹膜播散中的关系。方法30只经环孢素A处理的SD大鼠分成3组，分别向大鼠腹腔内接种经溴脱氧尿甙(BrdU)标记的Sy86B裸鼠可移植性人胃癌细胞，数量分别为105、106和107。应用免疫组织化学染色，观察癌细胞的分布。计数网膜乳斑或非乳斑区标记细胞的数量，确定癌细胞的分布。结果在乳斑或非乳斑区平均标记细胞数的比率分别是163∶1(105)、280∶1(106)和697∶1(107)。标记细胞数在乳斑或非乳斑区之间的差异有显著性意义(P<001)。结论标记细胞选择性地侵入网膜乳斑，表明在胃癌腹膜播散的早期，癌细胞具有特异地侵入腹膜乳斑的特性。
The relationship between peritoneal milkyspots and peritoneal dissemination of gastric carcinoma
AN Weide,HU Xiang,LIANG Pin,et al
(Department of Surgery,First Affiliated Hospital,Dalian University of MedicalSciences,Dalian 116011,China)
【 Abstract】 Objective To investigate the relationshipbetween peritoneal milky spots and peritoneal dissemination of gastric carcinoma. MethodsThirty SD rats treated with cyclosporin A were divided into 3 groups and inoculatedintroperitoneally with human gastric cancer sy86B cells labeled withbromodeoxyuridine(BrdU)at 105、 106 and 107 cells per rat.The distribution of the labeledcells in the greater omentum,taken from the rats using an anti BrdU antibody,wasobserved with immunohistochemical assay.The number of the BrdU labeled cell per unitarea at each milky spot and non milky spot in the omentum was counted.Results Theratios of the mean labeled cell numbers in the milky spot sites to that in the nonmilky spot sites were 163∶ 1(105) ......
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