关键词:细胞凋亡;Caspase-1基因;逆转录病毒载体;胃癌细胞;基因治疗
【摘要】 目的 探讨转染Caspase-1基因对胃癌细胞生物学特性的影响。方法 将人凋亡基因Caspase-1的重组逆转录病毒导入胃癌细胞株X-108,对转染阳性细胞的体外增殖、细胞培养液中CEA含量及裸鼠致瘤性进行分析。结果 导入的Caspase-1基因使细胞增殖周期发生变化,G1 期细胞数目增多,S期细胞数目减少。转染细胞培养液CEA含量有下降趋势。胃癌细胞株X-108-Caspase-1体外生长速度明显受抑,裸鼠体内致瘤性降低,肿瘤生长缓慢。结论 Caspase-1不能促使X-108胃癌细胞凋亡,但可以使其增殖受抑,肿瘤细胞的恶性程度降低。
Biologicalcharacteristics of the human gastric cancer
cell line transfected with Caspase-1 gene
XUE Xuchao,FANG Guoen,QIAN Qijun,et al. Changhai Hospital, Second Military MedicalUniversity, Shanghai 200433
【 Abstract】 Objective To investigate the biologicalcharacteristics of human gastric cancer cell transfected with Caspase-1 gene. Methods Recombinant retroviral vectorwith human Caspase-1 cDNA was transferred into human gastric cell line X-108. Theproliferation tumorigenicity, cycle of the delivered cell line and the CEA concentrationin the supernate of cell culture were analyzed respectively. Results Apoptosis was not induced ......
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