关键词:温和酸消化;血清;碘
摘 要:目的 测定血清中总碘含量。方法 温和酸消化法。结果 工作曲线在0~250μg/L范围内,r=-0.9985~-0.9998,最低检测浓度为15.86μg/L(取血清0.2ml)。标准及样品精密度变异系数均小于10%(n=6)。当血清碘本底为20.7,32.2,60.2μg/L,加标量为100μg/L时,回收率分别为98.47%,97.40%,102.57%(n=6)。结论 利用温和酸消化法测定血清总碘含量,结果较为理想。
中图分类号: R446.11;O613.44 文献标识码: A
文章编号: 1000-4955(2000)01-0071-02
Detection of total iodine in serum by acid-digesting under mild conditions
HU Mei,ZE Ren-yongxi
(Public Health & Anti-epidemic Center of Sichuan Province,Chengdu 610031,China)
Abstract:Objective To detect the total iodine in human serum.Methods Serum is digested by chloric acid under mild conditions.Results The coefficient of correlation of working curve are between -0.9985 and -0.9998 when the iodine contents are between 0 and 250μg/L.Using 0.2ml of serum,the lowest test concentration is 15.86μg/L.The variation coefficient of standards and samples are lower than 10%(n=6).The average rates of recovery are 98.47%,97.40%,102.57% when the serum iodine are 20.7,32.2,60.2μg/L,respectively,and added 100μg/L standard iodine.Conclusions Digesting the serum by acid under the mild conditions this method for detecting total iodine can get a good result.
Key words : Acid-digesting under mild conditions;Serum;Iodine▲
人体摄入碘的主要途径是通过食物经消化道转变成离子碘或小分子的含碘化合物后 ......
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