摘要 应用Southern印迹杂交和RT-PCR-SSCP银染、克隆及序列测定检测了36例大肠癌组织标本中nm23-H1杂合性缺失和突变情况。结果：本组标本nm23-H1杂合性缺失率为29.63%，Duke’s D期及远处转移大肠癌有较高的杂合性缺失发生率；大肠癌中未见nm23-H1基因突变。本实验结果提示： nm23-H1基因杂合性缺失可能参与大肠癌恶性进展及转移过程的调节，该基因突变在大肠癌中所起的作用可能较小。

    

THE RELATION OF THE LOSS OF HETEROZYGOSITY ANDMUTATION FOR nm23-H1 WITH THE INHIBITION OF METASTASIS IN COLORECTAL CARCINOMA

Luo Chengyu, Zhu Xueguang, Li Shiyong, et al.

    Department of General Surgery, Beijing Army General Hospital, Beijing 100700

Abstract The loss ofheterozygosity and mutation for nm23-H1 gene in colorectal carcinomas were studied bySouthern blot and RT-PCR-SSCP/silver staining sequencing. The rate of loss ofheterozygosity for nm23-H1 was 29.63%. The cases of Duke's stage D and distant metastatsishad higher frequency of the loss of heterozygosity. No mutation for nm23-H1 was found incolorectal carcinomas. These reaults indicate that the loss of heterozygosity for nm23-H1may play a significant role in the malignant progression and distant metastasis incolorectal carcinomas.

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