关键词：缺血/再灌注损伤；移植物动脉硬化；大鼠
摘 要：目的 探索缺血损伤在同品系大鼠腹主动脉移植硬化中的作用。方法 将90只大鼠随机分为3组：A组为同品系(Wistar to Wistar)移植缺血小于30分钟组；B组为同品系移植缺血4小时组；C组为异品系(SD to Wistar)移植缺血小于30分钟组。于移植术后15、30及60天切取植入的腹主动脉进行光镜和电镜检查，应用MIAS-300计算机图像分析系统自动测量移植腹主动脉管腔面积、内膜面积和中膜面积。结果 A组术后60天动脉内膜轻度增厚，但B组和C组术后60天均有明显管腔面积缩小和内膜增厚现象，与A组比较差异有显著性意义(P<0.05)。病理检查结果表明，B组和C组增生的内膜均由单核/巨噬细胞和平滑肌细胞构成，但前者无中膜层平滑肌坏死及弹力膜断裂现象。结论 缺血/再灌注损伤是移植动脉硬化的一个重要因素。
分类号： R617；R392.4 文献标识码： A
文章编号： 1007-9424(2000)01-0019-03
ISCHEMIA-INDUCED TRANSPLANT ARTERIOSCLEROSIS IN RATS AORTIC ISOGRAFT
WAN Yun-le,LI Bo,SHEN Wen-lü ,et al.
(Department of General Surgery,First Affiliated Hospital,West China University of Medical Sciences,Chengdu 610041)
Abstract：Objective To investigate the effect of cold ischemia on the development of transplant arteriosclerosis (TA) in rat aortic isografts.Methods Aorta grafts from SD and Wister rats were stored in a cold perfusion solution for 0.5 hours and 4 hours respectively before being orthotopically transplanted to Wister recipients.After observation times ranging from 15 to 60 days,the grafts were examined by using histological and electron microscopy techniques.Regional changes in the lumen,intima and media layers were measured by using an image analysis system.Results Partial intima thickings were showed in control isografts at 60 day post-transplantation.Pronounced intima thickings were seen in experimental isografts and control allografts at the same time.The thicking neointimas consist mainly of monocyte/macrophage and smooth muscle cells (SMC).The broken interior elastic lamina (IEL) and necrosis SMC in media were detected in allogenic grafts.Conclusion The damage due to prolonged cold ischemia time is sufficient to cause pronouced graft arteriosclerosis.
Key words ： Ischemia/reperfusion injury Transplant arteriosclerosis Rat▲
移植物缺血/再灌注损伤是影响移植物有功能存活的关键因素之一〔1〕 ......
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