AGS细胞系中12-LOX的表达及其抑制剂对细胞增殖的影响
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黄彩云, 陈丰霖, 李建英, 陈治新, 王小众
12-脂肪氧化酶;黄芩甙元;胃癌;增殖;凋亡;逆转录聚合酶链反应,黄彩云,陈丰霖,李建英,陈治新,王小众,通讯作者,:,Expressi
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黄彩云,陈丰霖,李建英, 陈治新, 王小众,福建医科大学附属协和医院消化内科 福建省福州市 350001
黄彩云,女,1979-02-26生,福建省漳州市人,汉族.2003年福建医科大学硕士生,主要从事胃肠道肿瘤的研究.
福建省教育厅基金项目,No. JA03094
通讯作者:陈丰霖,350001, 福建省福州市新权路29号,福建医科大学附属协和医院消化内科.
电话: 0591-83357896-8482
收稿日期:2005-04-11 接受日期:2005-05-14
Expression of12-lipoxygenase and its inhibitor's effect on proliferation of human AGScell line
Cai-Yun Huang, Feng-Lin Chen, Jian-Ying Li, Zhi-Xin Chen, Xiao-Zhong Wang
Cai-Yun Huang, Feng-Lin Chen, Jian-Ying Li, Zhi-Xin Chen, Xiao-ZhongWang, Department of Gastroenterology, Union Hospital, Fujian MedicalUniversity, Fuzhou 350001, Fujian Province, China
Supported by the Fund from Education Commision of Fujian Province,China, No. JA03094
Correspondence to: Feng-Lin Chen, Department of Gastroenterology,Union Hospital, Fujian Medical University, Fuzhou 350001, Fujian Province,China.
Received: 2005-04-11 Accepted:2005-05-14
Abstract
AIM: To investigate the expression of 12-lipoxygenase (12-LOX) andthe possible effect of its inhibitor on proliferation of human gastriccancer cell line AGS.
METHODS: AGS cells were cultured in RPMI-1640 medium with 100 mL/Lfetal bovine serum (FBS), 100 kU/L penicillin and 100 kU/L streptomycin.12-LOX mRNA was analyzed by reverse transcription polymerase chainreaction (RT-PCR). The effect of baicalein on the proliferation ofthe cells was detected by thiazolyl blue tetrazolium bromide (MTT) method.The morphological changes of cellular and subcellullar structures wereobserved under inverted microscope and electron microscopy. AO-EB doubleand TUNEL staining were used to evaluate the apoptotic index of AGS cells.
RESULTS: 12-LOX mRNA was expressed in human AGS cells. Atconcentrations from 20 to 80 mmol/L, baicalein inhibitedthe proliferation of AGS cells in a concentration- and time-dependentmanner from 24 to 72 hours. There were significant differences between anyother groups (P<0 ......
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